Literature summary for 1.14.14.102 extracted from

Frick, S.; Kramell, R.; Kutchan, T.M.
Metabolic engineering with a morphine biosynthetic P450 in opium poppy surpasses breeding (2007), Metab. Eng., 9, 169-176.

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Application

Application	Comment	Organism
biotechnology	the enzyme provides practical means to genetically engineer valuable secondary metabolites in this important medicinal plant	Papaver somniferum

Cloned(Commentary)

Cloned (Comment)	Organism
gene cyp80b3, overexpression in Papaver somniferum plants, expression of anti-sense constructs, construction of transgenic plants using the Agrobacterium tumefaciens, strain AGL1, transfection method, expression of antisense cyp80b3 leads to a reduction of total alkaloid in latex up to 84%, phenotype, overview, enzyme overexpression results in an up to 450% increase in the amount of total alkaloid in latex without changing the ratio of the individual alkaloids, or together with an overall increase in the ratio of morphine	Papaver somniferum

Cloned (Comment)

Organism

gene cyp80b3, overexpression in Papaver somniferum plants, expression of anti-sense constructs, construction of transgenic plants using the Agrobacterium tumefaciens, strain AGL1, transfection method, expression of antisense cyp80b3 leads to a reduction of total alkaloid in latex up to 84%, phenotype, overview, enzyme overexpression results in an up to 450% increase in the amount of total alkaloid in latex without changing the ratio of the individual alkaloids, or together with an overall increase in the ratio of morphine

Papaver somniferum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	a cytochrome P450 enzyme	Papaver somniferum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2	Papaver somniferum	the enzyme catalyzes a key regulation step in morphine biosynthesis	(S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Papaver somniferum	-	cv. C048-6-14-64	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2	-	Papaver somniferum	(S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2	the enzyme catalyzes a key regulation step in morphine biosynthesis	Papaver somniferum	(S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O	-	?

Synonyms

Synonyms	Comment	Organism
(S)-N-methylcoclaurine 3'-hydroxylase	-	Papaver somniferum
CYP80B3	-	Papaver somniferum

Cofactor

Cofactor	Comment	Organism	Structure
cytochrome P450	dependent on	Papaver somniferum