Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha cells | Aminobacter aminovorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Aminobacter aminovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50500 | - |
x * 50500, calculated from amino acid sequence | Aminobacter aminovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aminobacter aminovorans | P54989 | component A of NTA monooxygenase | - |
Aminobacter aminovorans ATCC 29600 | P54989 | component A of NTA monooxygenase | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.076 | - |
in 30 mM Tris-HCl (pH 8.0), 10 mM FMN, and 2 mM MgCl2, temperature not specified in the publication | Aminobacter aminovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nitrilotriacetate + FMNH2 + H+ + O2 | - |
Aminobacter aminovorans | iminodiacetate + glyoxylate + FMN + H2O | - |
ir | |
nitrilotriacetate + FMNH2 + H+ + O2 | - |
Aminobacter aminovorans ATCC 29600 | iminodiacetate + glyoxylate + FMN + H2O | - |
ir |
Subunits | Comment | Organism |
---|---|---|
? | x * 50500, calculated from amino acid sequence | Aminobacter aminovorans |
Synonyms | Comment | Organism |
---|---|---|
NTA monooxygenase | - |
Aminobacter aminovorans |
NTA-MO | - |
Aminobacter aminovorans |
NtaA | component A of NTA monooxygenase | Aminobacter aminovorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMNH2 | - |
Aminobacter aminovorans |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aminobacter aminovorans | calculated from amino acid sequence | - |
6.5 |