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Literature summary for 1.14.14.10 extracted from
- Cloning and characterization of the genes encoding nitrilotriacetate monooxygenase of Chelatobacter heintzii ATCC 29600 (1996), J. Bacteriol., 178, 6123-6132.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli DH5alpha cells | Aminobacter aminovorans |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Aminobacter aminovorans |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50500 | - |
x * 50500, calculated from amino acid sequence | Aminobacter aminovorans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aminobacter aminovorans | P54989 | component A of NTA monooxygenase | - |
| Aminobacter aminovorans ATCC 29600 | P54989 | component A of NTA monooxygenase | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.076 | - |
in 30 mM Tris-HCl (pH 8.0), 10 mM FMN, and 2 mM MgCl2, temperature not specified in the publication | Aminobacter aminovorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nitrilotriacetate + FMNH2 + H+ + O2 | - |
Aminobacter aminovorans | iminodiacetate + glyoxylate + FMN + H2O | - |
ir | |
| nitrilotriacetate + FMNH2 + H+ + O2 | - |
Aminobacter aminovorans ATCC 29600 | iminodiacetate + glyoxylate + FMN + H2O | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50500, calculated from amino acid sequence | Aminobacter aminovorans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NTA monooxygenase | - |
Aminobacter aminovorans |
| NTA-MO | - |
Aminobacter aminovorans |
| NtaA | component A of NTA monooxygenase | Aminobacter aminovorans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMNH2 | - |
Aminobacter aminovorans | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aminobacter aminovorans | calculated from amino acid sequence | - |
6.5 |
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