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Literature summary for 1.14.14.10 extracted from

  • Xun, L.; Reeder, R.; Plymale, A.; Girvin, D.; Bolton Jr., H.
    Degradation of metal - nitrilotriacetate complexes by nitrilotriacetate monooxygenase (1996), Environ. Sci. Technol., 30, 1752-1755.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ni2+ ionic or uncomplexed Ni2+ (2 mM) is inhibitory to the enzyme Aminobacter aminovorans
Zn2+ ionic or uncomplexed Zn2+ (2 mM) is inhibitory to the enzyme Aminobacter aminovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0317
-
nitrilotriacetate with equimolar Ni2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C Aminobacter aminovorans
0.0367
-
nitrilotriacetate with equimolar Co2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C Aminobacter aminovorans
0.0404
-
nitrilotriacetate with equimolar Mg2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C Aminobacter aminovorans
0.043
-
nitrilotriacetate with 2 mM Mg2+and varying nitrilotriacetate concentrations, in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C Aminobacter aminovorans
0.0576
-
nitrilotriacetate with equimolar Zn2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C Aminobacter aminovorans
0.0905
-
nitrilotriacetate with equimolar Mn2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C Aminobacter aminovorans
0.0968
-
nitrilotriacetate with equimolar Fe2+ (0.04-0.2 mM), in 20 mM HEPES buffer (pH 7.6), 0.01 mM FMN, at 24°C Aminobacter aminovorans

Metals/Ions

Metals/Ions Comment Organism Structure
Al3+ nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+ Aminobacter aminovorans
Co2+ nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+ Aminobacter aminovorans
Cu2+ nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+ Aminobacter aminovorans
Fe2+ nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+ Aminobacter aminovorans
Mg2+ the enzyme shows little preference for Mg-nitrilotriacetate over Co-nitrilotriacetate, Ni-nitrilotriacetate, and Zn-nitrilotriacetate Aminobacter aminovorans
Ni2+ nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+ Aminobacter aminovorans
Zn2+ nitrilotriacetate is only a substrate when complexed with cations such as Mg2+, Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+ Aminobacter aminovorans

Organism

Organism UniProt Comment Textmining
Aminobacter aminovorans
-
-
-
Aminobacter aminovorans ATCC 29600
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially purified by ammonium sulfate precipitation and phenyl agarose column chromatography Aminobacter aminovorans

Storage Stability

Storage Stability Organism
-80°C, partially purified, dialyzed enzyme, 3 months, no loss of activity Aminobacter aminovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information nitrilotriacetate not complexed with cation is not degraded. The addition of Cr2+,Ba2+, Sr2+, Cr3+, K+, or Na+ results in no degradation of nitrilotriacetate Aminobacter aminovorans ?
-
?
additional information nitrilotriacetate not complexed with cation is not degraded. The addition of Cr2+,Ba2+, Sr2+, Cr3+, K+, or Na+ results in no degradation of nitrilotriacetate Aminobacter aminovorans ATCC 29600 ?
-
?
nitrilotriacetate + FMNH2 + H+ + O2 nitrilotriacetate is only a substrate when complexed with cations such as Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+. The enzyme shows little preference for Mg-nitrilotriacetate over Co-nitrilotriacetate, Ni-nitrilotriacetate, and Zn-nitrilotriacetate Aminobacter aminovorans iminodiacetate + glyoxylate + FMN + H2O
-
?
nitrilotriacetate + FMNH2 + H+ + O2 nitrilotriacetate is only a substrate when complexed with cations such as Al3+, Cu2+, Ni2+, Zn2+, Fe2+ or Co2+. The enzyme shows little preference for Mg-nitrilotriacetate over Co-nitrilotriacetate, Ni-nitrilotriacetate, and Zn-nitrilotriacetate Aminobacter aminovorans ATCC 29600 iminodiacetate + glyoxylate + FMN + H2O
-
?

Synonyms

Synonyms Comment Organism
NTA-MO
-
Aminobacter aminovorans

Cofactor

Cofactor Comment Organism Structure
FMNH2
-
Aminobacter aminovorans