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Literature summary for 1.14.14.1 extracted from

  • Zhu, Y.; Nomura, T.; Xu, Y.; Zhang, Y.; Peng, Y.; Mao, B.; Hanada, A.; Zhou, H.; Wang, R.; Li, P.; Zhu, X.; Mander, L.N.; Kamiya, Y.; Yamaguchi, S.; He, Z.
    Elongated uppermost internode encodes a cytochrome P450 monooxygenase that epoxidizes gibberellins in a novel deactivation reaction in rice (2006), Plant Cell, 18, 442-456.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
biotechnology EUI and the GA metabolism pathway are useful targets for increasing the agronomic value of crops Oryza sativa

Cloned(Commentary)

Cloned (Comment) Organism
gene eui, i.e. elongated uppermost internode, map-based cloning, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of EUI in Saccharomyces cerevisiae strain WAT11 microsomes, expression of a chimeric EUI–GFP in onion epidermic cells with localization in the cytoplasm on the surface of the endoplamic reticulum Oryza sativa

Protein Variants

Protein Variants Comment Organism
additional information construction of the recessive elongated uppermost internode tall mutant, which is morphologically normal until its final internode elongates drastically at the heading stage, the stage-specific developmental effect of the eui mutation is used in the breeding of hybrid rice to improve the performance of heading in male sterile cultivars, the eui mutant accumulates exceptionally large amounts of biologically active gibberellins in the uppermost internode, eui mutant plants and Eui-overexpressing transgenic plants show tall and dwarfed phenotypes, and fail to produce seeds, overview Oryza sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum the EUI protein is anchored on the endoplasmic reticulum towards the cytoplasm Oryza sativa 5783
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Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ heme iron Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
giberellic acid GA4 + [reduced NADPH-hemoprotein reductase] + O2 Oryza sativa
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16alpha,17-epoxy GA4 + [oxidized NADPH-hemoprotein reductase] + H2O the product is transformed further to 16,17-dihydro-16alpha,17-dihydroxy-GA4 in the plant ?
additional information Oryza sativa EUI acts as a gibberellic acid inactivating enzyme, in eui mutant plans, the biological activity of GA4 is reduced, expression of Eui is tightly regulated during plant development with the stage-specific eui phenotypes, GA biosynthesis and catabolism pathways, overview, the enzyme negatively regulates gibberellic acid metabolism, mechanism, overview ?
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?

Organism

Organism UniProt Comment Textmining
Oryza sativa Q5KQH7 ssp. japonica, wild-type strain ZS97, gene eui or OSJNBa0095J22.13
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Source Tissue

Source Tissue Comment Organism Textmining
additional information expression of Eui is tightly regulated during plant development with the stagespecific eui phenotypes, overview Oryza sativa
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
giberellic acid GA12 + [reduced NADPH-hemoprotein reductase] + O2
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Oryza sativa 16alpha,17-epoxy GA12 + [oxidized NADPH-hemoprotein reductase] + H2O product identification by GC-MS, the epoxid is transformed into 16,17-dihydro-16alpha,17-dihydroxy-GA4 under acidic conditions with acidic acid ?
giberellic acid GA4 + [reduced NADPH-hemoprotein reductase] + O2
-
Oryza sativa 16alpha,17-epoxy GA4 + [oxidized NADPH-hemoprotein reductase] + H2O the product is transformed further to 16,17-dihydro-16alpha,17-dihydroxy-GA4 in the plant ?
giberellic acid GA4 + [reduced NADPH-hemoprotein reductase] + O2
-
Oryza sativa 16alpha,17-epoxy GA4 + [oxidized NADPH-hemoprotein reductase] + H2O product identification by GC-MS, the epoxid is transformed into 16,17-dihydro-16alpha,17-dihydroxy-GA4 under acidic conditions with acidic acid ?
giberellic acid GA9 + [reduced NADPH-hemoprotein reductase] + O2
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Oryza sativa 16alpha,17-epoxy GA9 + [oxidized NADPH-hemoprotein reductase] + H2O product identification by GC-MS, the epoxid is transformed into 16,17-dihydro-16alpha,17-dihydroxy-GA4 under acidic conditions with acidic acid ?
additional information EUI acts as a gibberellic acid inactivating enzyme, in eui mutant plans, the biological activity of GA4 is reduced, expression of Eui is tightly regulated during plant development with the stage-specific eui phenotypes, GA biosynthesis and catabolism pathways, overview, the enzyme negatively regulates gibberellic acid metabolism, mechanism, overview Oryza sativa ?
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?
additional information EUI catalyzes the 16alpha,17-epoxidation of non-13-hydroxylated gibberellic acids with very high activity on giberellic acid GA4, substrate specificity, overview, no activity with the 13-hydroxylated GA1, GA20, and GA53 Oryza sativa ?
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?

Synonyms

Synonyms Comment Organism
CYP714D1
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Oryza sativa
cytochrome P450 monooxygenase
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Oryza sativa
EUI
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Oryza sativa
GA 16a,17-epoxidase
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Oryza sativa
GA-deactivating enzyme
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Oryza sativa
More EUI Is a member of the CYP714 family Oryza sativa
Os05g0482400 protein
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Oryza sativa

Cofactor

Cofactor Comment Organism Structure
NADPH
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Oryza sativa