Application | Comment | Organism |
---|---|---|
additional information | the enzyme is a target for improving the catalytic performance of P450 BM-3 toward nonnatural substrates of industrial importance in the presence of organic solvents or cosolvents for industrial applications | Priestia megaterium |
Protein Variants | Comment | Organism |
---|---|---|
F87A | site-directed mutagenesis, the mutant exhibits an altered regioselectivity and substrate specificity compared with wild-type, it has lower tolerance toward DMSO | Priestia megaterium |
additional information | the enzyme is a target for improving the catalytic performance of P450 BM-3 toward nonnatural substrates of industrial importance in the presence of organic solvents or cosolvents for industrial applications | Priestia megaterium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | in a catalytic cytochrome P450 ferric heme center | Priestia megaterium |
Organic Solvent | Comment | Organism |
---|---|---|
DMSO | inactivation mechanism, the mutant F87A is more sensitive to DMSO than the wild-type enzyme due to the absence of the phenyl ring in the mutant which promotes interactions of the DMSO molecule with the heme iron resulting in water displacement by DMSO at the catalytic heme center, box by stacking equilibrated boxes of solvent molecules to, modeling of DMSO in the active site channel, overview | Priestia megaterium |
additional information | inactivation mechanism of monooxygenase P450 BM-3 by organic cosolvents: a molecular dynamics simulation study using the X-ray P450 BM-3 crystal structure, PDB code 1BU7 | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | P14779 | - |
- |
Subunits | Comment | Organism |
---|---|---|
More | structure alteration due to organic solvents, analysis by molecular dynamics simulation study using the X-ray P450 BM-3 crystal structure, PDB code 1BU7 | Priestia megaterium |
Synonyms | Comment | Organism |
---|---|---|
monooxygenase P450 BM-3 | - |
Priestia megaterium |