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Literature summary for extracted from

  • Purwani, N.N.; Martin, C.; Savino, S.; Fraaije, M.W.
    Modular assembly of phosphite dehydrogenase and phenylacetone monooxygenase for tuning cofactor regeneration (2021), Biomolecules, 11, 905 .
    View publication on PubMedView publication on EuropePMC


Cloned (Comment) Organism
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli Thermobifida fusca

Protein Variants

Protein Variants Comment Organism
additional information addition of the dimerization-docking and anchoring domain (RIDD-RIAD) system to the C-terminus of the NADPH-dependent Baeyer-Villiger monooxygenase phenylacetone monooxygenase (PAMO) and the NADPH-regenerating enzyme (phosphite dehydrogenase, PTDH, EC allowing self-assembly based on specific protein-protein interactions between both peptides and allow tuning of the ratio of the targeted enzymes as the RIAD peptide binds to two RIDD peptides. Several RIDD/RIAD-tagged PAMO and PTDH variants are successfully overproduced in Escherichia coli and subsequently purified. Complementary tagged enzymes are mixed and analyzed for their oligomeric state, stability, and activity. Complexes are formed in the case of some specific combinations (PAMORIAD-PTDHRIDD and PAMORIAD/RIAD-PTDHRIDD). These enzyme complexes display similar catalytic activity when compared with the PTDH-PAMO fusion enzyme. The thermostability of PAMO in these complexes is retained while PTDH displays somewhat lower thermostability Thermobifida fusca

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phenylacetone + NADPH + H+ + O2 Thermobifida fusca
benzyl acetate + NADP+ + H2O


Organism UniProt Comment Textmining
Thermobifida fusca Q47PU3

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
phenylacetone + NADPH + H+ + O2
Thermobifida fusca benzyl acetate + NADP+ + H2O


Synonyms Comment Organism
Thermobifida fusca

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
assay at Thermobifida fusca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
assay at Thermobifida fusca


Cofactor Comment Organism Structure
Thermobifida fusca
Thermobifida fusca

General Information

General Information Comment Organism
evolution phenylacetone monooxygenase, PAMO, is a NADPH-dependent Baeyer-Villiger monooxygenase Thermobifida fusca