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Literature summary for 1.14.13.92 extracted from

  • Gran-Scheuch, A.; Parra, L.; Fraaije, M.
    Systematic assessment of uncoupling in flavoprotein oxidases and monooxygenases (2021), ACS Sust. Chem. Eng., FEHLT, 0000 .
No PubMed abstract available

Protein Variants

Protein Variants Comment Organism
C65D site-directed mutagenesis, the engineered TfPAMO variant acts as an NADPH oxidase, it shows an extremely high rate of uncoupling compared with the wild-type enzyme. The mutant is not effective in stabilizing the C(4alpha)-peroxyflavin intermediate. For TfPAMO C65D, hydrogen peroxide and superoxide levels are highest at pH 9.0 Thermobifida fusca

Inhibitors

Inhibitors Comment Organism Structure
additional information no detrimental effect of the accumulation of superoxide on biocatalysis is demonstrated Thermobifida fusca

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phenylacetone + NADPH + H+ + O2 Thermobifida fusca
-
benzyl acetate + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Thermobifida fusca Q47PU3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information for wild-type TfPAMO, the uncoupling side activity at different pHs ranges from 5.0 to 7.8%. Formation of hydrogen peroxide is rather constant (around 4.0 microM), while formation of superoxide is almost absent at low pH of 6.0, reaching 3.0 microM at high pH of 9.0-10.0 Thermobifida fusca ?
-
-
phenylacetone + NADPH + H+ + O2
-
Thermobifida fusca benzyl acetate + NADP+ + H2O
-
?

Synonyms

Synonyms Comment Organism
PAMO
-
Thermobifida fusca
Tf PAMO
-
Thermobifida fusca

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
25
-
assay at Thermobifida fusca

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Thermobifida fusca

pH Range

pH Minimum pH Maximum Comment Organism
6 10 activity range, profile overview Thermobifida fusca

Cofactor

Cofactor Comment Organism Structure
FAD
-
Thermobifida fusca
NADPH
-
Thermobifida fusca

General Information

General Information Comment Organism
evolution phenylacetone monooxygenase from Thermobifida fusca (TfPAMO) is a thermostable Baeyer-Villiger monooxygenase from the NAD(P)H/FAD-dependent oxidoreductase family Thermobifida fusca