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Literature summary for 1.14.13.92 extracted from

  • de Gonzalo, G.; Torres Pazmino, D.E.; Ottolina, G.; Fraaije, M.W.; Carrea, G.
    Oxidations catalyzed by phenylacetone monooxygenase from Thermobifida fusca (2005), Tetrahedron, 16, 3077-3083.
No PubMed abstract available

Application

Application Comment Organism
synthesis the enzyme has catalytic potential as a biocatalyst Thermobifida fusca

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetics Thermobifida fusca

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
phenylacetone + NADPH + H+ + O2 Thermobifida fusca
-
benzyl acetate + NADP+ + H2O
-
?

Organism

Organism UniProt Comment Textmining
Thermobifida fusca
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
additional information
-
substrate specificity, diverse substrates, overview Thermobifida fusca

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-1-acetoxy-phenylacetone + NADPH + O2
-
Thermobifida fusca (R)-1-hydroxy-1-phenylacetone + NADP+ + H2O
-
?
(S)-nicotine + NADPH + O2
-
Thermobifida fusca ?
-
?
3-phenylpenta-2,4-dione + NADPH + O2
-
Thermobifida fusca (R)-phenylacetylcarbinol + NADP+ + H2O the product is a well-known precursor in the synthesis of ephedrine and pseudoephedrine ?
benzylacetone + NADPH + O2 low activity Thermobifida fusca ?
-
?
diketone + NADPH + O2
-
Thermobifida fusca (R)-1-acetoxy-phenylacetone + NADP+ + H2O
-
?
additional information the recombinant His-tagged enzyme is active with a large range of sulfides and ketones, as well as with several sulfoxides, an amine and an organoboron compound, high enantioselectivity dependeing on the substrate, substrate specificity, overview Thermobifida fusca ?
-
?
N,N-dimethylbenzylamine + NADPH + O2
-
Thermobifida fusca N,N-dimethylbenzylamine N-oxide + NADP+ + H2O
-
?
phenylacetone + NADPH + H+ + O2
-
Thermobifida fusca benzyl acetate + NADP+ + H2O
-
?
phenylboronic acid + NADPH + O2 formation of phenol Thermobifida fusca ?
-
?

Synonyms

Synonyms Comment Organism
More the enzyme belongs to the Baeyer-Villiger monooxygenases Thermobifida fusca
PAMO
-
Thermobifida fusca

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
25
-
assay at Thermobifida fusca

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information all substrates show a turnover between 1.2 s-1 and 3.6 s-1 Thermobifida fusca

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Thermobifida fusca