Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determnination and analysis, sequence comparisons, expression of FLAG-tagged wild-type enzyme and of C-terminal truncation mutants in COS-7 cells. The FLAG tag directs the wild-type enzyme to mitochondria but also to the cytosol and the plasma mambrane, overview | Sus scrofa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of C-terminal truncation mutants KMODELTAC10, KMODELTAC20, KMODELTAC30, KMODELTAC50, and KMODELTAC70, that are all localized in the cytosol after recombinant expression in COS-7 cells, exept for KMODELTAC10. Two forms of C-terminal truncation, KMODELTAC10D and KMODELTAC20D retain almost full activity but KMODELTAC30D shows 1.6fold higher activity than the wild-type. The activities of KMODELTAC50 and KMODELTAC70 is highly reduced, overview. The quantity of expressed KMODELTA30D in COS-7 cellsis 1.3fold higher than the wild-type KMO | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial outer membrane | the C-terminal region functions as a mitochondrial targeting signal | Sus scrofa | 5741 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-kynurenine + NADH + H+ + O2 | Sus scrofa | - |
3-hydroxy-L-kynurenine + NAD+ + H2O | - |
? | |
L-kynurenine + NADPH + H+ + O2 | Sus scrofa | - |
3-hydroxy-L-kynurenine + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | Q9MZS9 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-kynurenine + NADH + H+ + O2 | - |
Sus scrofa | 3-hydroxy-L-kynurenine + NAD+ + H2O | - |
? | |
L-kynurenine + NADPH + H+ + O2 | - |
Sus scrofa | 3-hydroxy-L-kynurenine + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the C-terminal region is required for the enzymatic activity and functions as a mitochondrial targeting signal | Sus scrofa |
Synonyms | Comment | Organism |
---|---|---|
KMO | - |
Sus scrofa |
L-kynurenine 3-monooxygenase | - |
Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Sus scrofa | |
NADH | - |
Sus scrofa | |
NADPH | - |
Sus scrofa |
General Information | Comment | Organism |
---|---|---|
additional information | the C-terminal region of pig liver KMO plays a dual role. First, it is required for the enzymatic activity. Second, it functions as a mitochondrial targeting signal | Sus scrofa |