Application | Comment | Organism |
---|---|---|
molecular biology | wild-type KMO gene can be used as a marker gene for visually screening transgenic silkworms | Bombyx mori |
molecular biology | the wild-type enzyme gene can be used as a marker gene for visually screening transgenic silkworms | Bombyx mori |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme deficiency leads to the white eyes and white eggs w-1 phenotype in mutant silkworms, it can be partially rescued by expression of wild-type enzyme under control of either the cytoplasmic actin gene promoter, A3KMO, or the native KMO gene promoter, KKMO, phenotypes, overview | Bombyx mori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-kynurenine + NADPH + H+ + O2 | Bombyx mori | - |
3-hydroxy-L-kynurenine + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bombyx mori | - |
- |
- |
Bombyx mori | Q95NK3 | - |
- |
Bombyx mori C108 | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fat body | - |
Bombyx mori | - |
fat body | enzyme expression | Bombyx mori | - |
larva | - |
Bombyx mori | - |
malpighian tubule | - |
Bombyx mori | - |
malpighian tubule | enzyme expression | Bombyx mori | - |
midgut | - |
Bombyx mori | - |
midgut | enzyme expression | Bombyx mori | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-kynurenine + NADPH + H+ + O2 | - |
Bombyx mori | 3-hydroxy-L-kynurenine + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KMO | - |
Bombyx mori |
General Information | Comment | Organism |
---|---|---|
physiological function | the white egg 1 mutant, which is characterized by white eyes and white eggs, is deficient in Bombyx kynurenine 3-monooxygenase activity.Expression of the wild-type gene under control of either the cytoplasmic actin gene promoter (A3KMO) or the native KMO gene promoter (KKMO) leads to adults with brown eyes, and the eggs laid by the transgenic females are also brown. The A3KMO silkworm lines express the transcript in the mid-gut, fat bodies, and Malpighian tubules. The KKMO line expresses the transcript only in the fat bodies and Malpighian tubules. The intensity of eye and egg color in the transgenic lines is proportional to the KMO expression level | Bombyx mori |