Activating Compound | Comment | Organism | Structure |
---|---|---|---|
azide | 0.005 M | Rattus norvegicus | |
thiocyanate | 0.01 M | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CN- | inhibition at 0.01 M | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
NADPH | - |
Rattus norvegicus | |
0.025 | - |
kynurenine | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Br- | strong stimulation | Rattus norvegicus | |
Cl- | strong stimulation at 0.02 M | Rattus norvegicus | |
CN- | stimulates | Rattus norvegicus | |
F- | stimulates | Rattus norvegicus | |
I- | stimulates | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-kynurenine + NADPH + O2 | Rattus norvegicus | - |
? + NADP+ | - |
? | |
L-kynurenine + NADPH + O2 | Rattus norvegicus Osborne-Mendel | - |
? + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Osborne-Mendel strain | - |
Rattus norvegicus Osborne-Mendel | - |
Osborne-Mendel strain | - |
Purification (Comment) | Organism |
---|---|
partial | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-kynurenine + NADPH + O2 | no reaction with D-isomer | Rattus norvegicus | 3-hydroxy-L-kynurenine + NADP+ + H2O | - |
? | |
L-kynurenine + NADPH + O2 | no reaction with D-isomer | Rattus norvegicus Osborne-Mendel | 3-hydroxy-L-kynurenine + NADP+ + H2O | - |
? | |
L-kynurenine + NADPH + O2 | - |
Rattus norvegicus | ? + NADP+ | - |
? | |
L-kynurenine + NADPH + O2 | - |
Rattus norvegicus Osborne-Mendel | ? + NADP+ | - |
? | |
o-hydroxybenzoyl-DL-alanine + NADPH + O2 | about 25% of the activity with L-kynurenine | Rattus norvegicus | ? + NADP+ | - |
? | |
o-hydroxybenzoyl-DL-alanine + NADPH + O2 | about 25% of the activity with L-kynurenine | Rattus norvegicus Osborne-Mendel | ? + NADP+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9.3 | about 50% of maximal activity at pH 7 and 9.3 | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Rattus norvegicus | |
NADPH | - |
Rattus norvegicus |