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Literature summary for 1.14.13.82 extracted from

  • Providenti, M.A.; OBrien, J.M.; Ruff, J.; Cook, A.M.; Lambert, I.B.
    Metabolism of isovanillate, vanillate, and veratrate by Comamonas testosteroni strain BR6020 (2006), J. Bacteriol., 188, 3862-3869.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information the enzyme is inducible by vanillic acid and veratric acid Comamonas testosteroni

Cloned(Commentary)

Cloned (Comment) Organism
gene ivaA, DNA and amino acid sequence determination and analysis, genetic organization, physical map, expression analysis, recombinant expression in Escherichia coli Comamonas testosteroni
gene ivaB, DNA and amino acid sequence determination and analysis, genetic organization, physical map, expression analysis, recombinant expression in Escherichia coli Comamonas testosteroni
gene vanA, DNA and amino acid sequence determination and analysis, genetic organization, physical map, expression analysis, recombinant expression in Escherichia coli Comamonas testosteroni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isovanillic acid + NAD(P)H + H+ + O2 Comamonas testosteroni O-demethylation by IvaAB protocatechuic acid + NAD(P)+ + H2O + formaldehyde
-
?
isovanillic acid + NAD(P)H + H+ + O2 Comamonas testosteroni BR6020 O-demethylation by IvaAB protocatechuic acid + NAD(P)+ + H2O + formaldehyde
-
?
vanillic acid + NAD(P)H + H+ + O2 Comamonas testosteroni O-demethylation by VanA protocatechuic acid + NAD(P)+ + H2O + formaldehyde
-
?
vanillic acid + NAD(P)H + H+ + O2 Comamonas testosteroni BR6020 O-demethylation by VanA protocatechuic acid + NAD(P)+ + H2O + formaldehyde
-
?
veratric acid + NAD(P)H + H+ + O2 Comamonas testosteroni O-demethylation by VanA isovanillic acid + NAD(P)+ + H2O + formaldehyde
-
?
veratric acid + NAD(P)H + H+ + O2 Comamonas testosteroni O-demethylation by IvaAB vanillic acid + NAD(P)+ + H2O + formaldehyde
-
?
veratric acid + NAD(P)H + H+ + O2 Comamonas testosteroni BR6020 O-demethylation by IvaAB vanillic acid + NAD(P)+ + H2O + formaldehyde
-
?
veratric acid + NAD(P)H + H+ + O2 Comamonas testosteroni BR6020 O-demethylation by VanA isovanillic acid + NAD(P)+ + H2O + formaldehyde
-
?

Organism

Organism UniProt Comment Textmining
Comamonas testosteroni Q2KQ78 gene ivaB; genes ivaA, and ivaB
-
Comamonas testosteroni Q2KQ79 gene ivaA; genes ivaA, and ivaB
-
Comamonas testosteroni Q2KQ82 gene vanA; gene vanA
-
Comamonas testosteroni BR6020 Q2KQ78 gene ivaB; genes ivaA, and ivaB
-
Comamonas testosteroni BR6020 Q2KQ79 gene ivaA; genes ivaA, and ivaB
-
Comamonas testosteroni BR6020 Q2KQ82 gene vanA; gene vanA
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information in succinate- and procatechuic acid-grown cells, there is negligible degradative activity towards vanillic acid, veratric acid, and isovanillic acid and little to no expression of genes van, ivaA, and ivaB, growth on vanillic acid or veratric acid results in production of active oxygenases and expression of vanA, ivaA, and ivaB Comamonas testosteroni
-
additional information in succinate- and procatechuic acid-grown cells, there is negligible degradative activity towards vanillic acid, veratric acid, and isovanillic acid and little to no expression of genes vanA, ivaA, and ivaB, growth on vanillic acid or veratric acid results in production of active oxygenases and expression of vanA, ivaA, and ivaB Comamonas testosteroni
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isovanillic acid + NAD(P)H + H+ + O2 O-demethylation by IvaAB Comamonas testosteroni protocatechuic acid + NAD(P)+ + H2O + formaldehyde
-
?
isovanillic acid + NAD(P)H + H+ + O2 i.e. 3-hydroxy-4-methoxybenzoate, O-demethylation by IvaAB Comamonas testosteroni protocatechuic acid + NAD(P)+ + H2O + formaldehyde procatechuic acid is 3,4-dihydroxybenzoate ?
isovanillic acid + NAD(P)H + H+ + O2 O-demethylation by IvaAB Comamonas testosteroni BR6020 protocatechuic acid + NAD(P)+ + H2O + formaldehyde
-
?
isovanillic acid + NAD(P)H + H+ + O2 i.e. 3-hydroxy-4-methoxybenzoate, O-demethylation by IvaAB Comamonas testosteroni BR6020 protocatechuic acid + NAD(P)+ + H2O + formaldehyde procatechuic acid is 3,4-dihydroxybenzoate ?
vanillic acid + NAD(P)H + H+ + O2 O-demethylation by VanA Comamonas testosteroni protocatechuic acid + NAD(P)+ + H2O + formaldehyde
-
?
vanillic acid + NAD(P)H + H+ + O2 i.e. 4-hydroxy-3-methoxybenzoate, O-demethylation by VanA Comamonas testosteroni protocatechuic acid + NAD(P)+ + H2O + formaldehyde procatechuic acid is 3,4-dihydroxybenzoate ?
vanillic acid + NAD(P)H + H+ + O2 O-demethylation by VanA Comamonas testosteroni BR6020 protocatechuic acid + NAD(P)+ + H2O + formaldehyde
-
?
vanillic acid + NAD(P)H + H+ + O2 i.e. 4-hydroxy-3-methoxybenzoate, O-demethylation by VanA Comamonas testosteroni BR6020 protocatechuic acid + NAD(P)+ + H2O + formaldehyde procatechuic acid is 3,4-dihydroxybenzoate ?
veratric acid + NAD(P)H + H+ + O2 O-demethylation by VanA Comamonas testosteroni isovanillic acid + NAD(P)+ + H2O + formaldehyde
-
?
veratric acid + NAD(P)H + H+ + O2 i.e. 3,4-dimethoxybenzoate, O-demethylation by VanA Comamonas testosteroni isovanillic acid + NAD(P)+ + H2O + formaldehyde
-
?
veratric acid + NAD(P)H + H+ + O2 O-demethylation by VanA Comamonas testosteroni BR6020 isovanillic acid + NAD(P)+ + H2O + formaldehyde
-
?
veratric acid + NAD(P)H + H+ + O2 i.e. 3,4-dimethoxybenzoate, O-demethylation by VanA Comamonas testosteroni BR6020 isovanillic acid + NAD(P)+ + H2O + formaldehyde
-
?
veratric acid + NAD(P)H + H+ + O2 O-demethylation by IvaAB Comamonas testosteroni vanillic acid + NAD(P)+ + H2O + formaldehyde
-
?
veratric acid + NAD(P)H + H+ + O2 i.e. 3,4-dimethoxybenzoate, O-demethylation by IvaAB Comamonas testosteroni vanillic acid + NAD(P)+ + H2O + formaldehyde
-
?
veratric acid + NAD(P)H + H+ + O2 O-demethylation by IvaAB Comamonas testosteroni BR6020 vanillic acid + NAD(P)+ + H2O + formaldehyde
-
?
veratric acid + NAD(P)H + H+ + O2 i.e. 3,4-dimethoxybenzoate, O-demethylation by IvaAB Comamonas testosteroni BR6020 vanillic acid + NAD(P)+ + H2O + formaldehyde
-
?

Subunits

Subunits Comment Organism
dimer presence of both subunits is essential Comamonas testosteroni

Synonyms

Synonyms Comment Organism
IvaAB
-
Comamonas testosteroni
More the enzyme belongs to the phthalate family oxygenases Comamonas testosteroni
VanA
-
Comamonas testosteroni

Cofactor

Cofactor Comment Organism Structure
NAD(P)H
-
Comamonas testosteroni