Literature summary for 1.14.13.81 extracted from

Bang, W.Y.; Jeong, I.S.; Kim, D.W.; Im, C.H.; Ji, C.; Hwang, S.M.; Kim, S.W.; Son, Y.S.; Jeong, J.; Shiina, T.; Bahk, J.D.
Role of Arabidopsis CHL27 protein for photosynthesis, chloroplast development and gene expression profiling (2008), Plant Cell Physiol., 49, 1350-1363.

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Cloned(Commentary)

Cloned (Comment)	Organism
PCR-amplification of cDNA from isolated RNA, PCR-products of enzyme-GFP fusion constructs are cloned into vector pPENSOTG and introduced into Arabidopsis protoplasts by polyethylene glycol-mediated transformation, complementation constructs are produced by joining PCR fragments of the enzyme promoter with the enzyme-GFP cDNA, cloned into vector pBIB-HYG, introduced into Agrobacterium GV3101, transformed into homozygous mutant plants via the floral dipping method	Arabidopsis thaliana

Cloned (Comment)

Organism

PCR-amplification of cDNA from isolated RNA, PCR-products of enzyme-GFP fusion constructs are cloned into vector pPENSOTG and introduced into Arabidopsis protoplasts by polyethylene glycol-mediated transformation, complementation constructs are produced by joining PCR fragments of the enzyme promoter with the enzyme-GFP cDNA, cloned into vector pBIB-HYG, introduced into Agrobacterium GV3101, transformed into homozygous mutant plants via the floral dipping method

Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
additional information	mutant plants are complemented with enzyme construct fused to GFP at C-terminus under native promoter or a constitutive 35S-promoter control, suppressing the mutant phenotype, N-terminal deletion of the first 39 amino acids leads to location in the cytosol instead of chloroplast membrane (crucial localization signal)	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast membrane	N-terminal 39 amino acids are essential for chloroplast localization of the enzyme	Arabidopsis thaliana	31969	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2	Arabidopsis thaliana	-	protochlorophyllide a + NADP+ + H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9M591	chl27-t knock-down mutant	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
magnesium-protoporphyrin IX monomethyl ester + NADPH + H+ + O2	-	Arabidopsis thaliana	protochlorophyllide a + NADP+ + H2O	-	?

Synonyms

Synonyms	Comment	Organism
aerobic Mg-protoporphyrin IX monomethyl ester cyclase	-	Arabidopsis thaliana
CHL27	-	Arabidopsis thaliana

General Information

General Information	Comment	Organism
malfunction	the Arabidopsis chl27-t knock-down mutant has a T-DNA insertion within the promoter region reducing mRNA levels dramatically, growth is slowed down and plants look pale due to chloroplast development defects (unstacking of thylakoid membranes), chlorophyll fluorescence analysis shows a low photosynthetic activity, numerous nuclear genes involved in pigment biosynthesis, photosynthesis (light-harvesting complex I and II, photo system I and II), for iron acquisition, carbon fixation, cold acclimation, and for electron carriers are repressed, stress and detoxifying related gene expression is affected most prominently, more than 600 genes are repressed, a similar number of genes are induced	Arabidopsis thaliana
metabolism	enzyme is involved in the protochlorophyllide a synthesis	Arabidopsis thaliana