Cloned (Comment) | Organism |
---|---|
gene xantha-1, DNA and amino acid sequence determination and analysis, expression of wild-type and mutants in transgenic barley plants | Hordeum vulgare |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of xantha-l35, xantha-l81, xantha-l82, viridis-k23, and viridis-k170 mutants, the mutants are all inactive in cyclase activity, phenotypes of mutant plants, overview | Hordeum vulgare |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Hordeum vulgare | 9507 | - |
membrane | the aerobic cyclase is composed of three gene products, a soluble protein, a membrane-bound component encoded by xantha-l, and another membrane-bound component encoded by viridis-k | Hordeum vulgare | 16020 | - |
soluble | the aerobic cyclase is composed of three gene products, a soluble protein, a membrane-bound component encoded by xantha-l, and another membrane-bound component encoded by viridis-k | Hordeum vulgare | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Hordeum vulgare |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2 | Hordeum vulgare | the enzyme is involved in the chlorophyll biosynthesis | magnesium-protochlorophyllide + NADP+ + H2O | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | Q5EFU4 | gene xantha-I; cv. Bonus, the aerobic cyclase is composed of three gene products, a soluble protein, a membrane-bound component encoded by xantha-l, and another membrane-bound component encoded by viridis-k | - |
Purification (Comment) | Organism |
---|---|
partially by chloroplast supernatant and membrane preparation of wild-type and recombinant mutant plants | Hordeum vulgare |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Hordeum vulgare | - |
seedling | - |
Hordeum vulgare | - |
stem | - |
Hordeum vulgare | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000085 | - |
average wild-type activity in chloroplasts | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2 | - |
Hordeum vulgare | magnesium-protochlorophyllide + NADP+ + H2O | - |
ir | |
magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + H+ + O2 | the enzyme is involved in the chlorophyll biosynthesis | Hordeum vulgare | magnesium-protochlorophyllide + NADP+ + H2O | - |
ir |
Subunits | Comment | Organism |
---|---|---|
More | the aerobic cyclase is composed of three different subunits: a soluble protein, a membrane-bound component encoded by Xantha-l, and another membrane-bound component encoded by viridis-k | Hordeum vulgare |
Synonyms | Comment | Organism |
---|---|---|
aerobic Mg-protoporphyrin IX monomethyl ester cyclase | - |
Hordeum vulgare |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Hordeum vulgare |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Hordeum vulgare |