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Literature summary for 1.14.13.8 extracted from

  • Ameria, S.P.; Jung, H.S.; Kim, H.S.; Han, S.S.; Kim, H.S.; Lee, J.H.
    Characterization of a flavin-containing monooxygenase from Corynebacterium glutamicum and its application to production of indigo and indirubin (2015), Biotechnol. Lett., 37, 1637-1644 .
    View publication on PubMed
Search for more literature for 1.14.13.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Top10 cells Corynebacterium glutamicum

General Stability

General Stability Organism
the enzyme activity is decreased to about 2.5-7 % at pH 8.0 in Tris/HCl or Tricine buffer Corynebacterium glutamicum

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ the enzyme is strongly inhibited by 1 mM Cu2+ Corynebacterium glutamicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.38
-
 Thiourea at pH 8.0 and 25°C Corynebacterium glutamicum
0.58
-
 trimethylamine at pH 8.0 and 25°C Corynebacterium glutamicum
6
-
 cysteamine at pH 8.0 and 25°C Corynebacterium glutamicum

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum
-
-
-
Corynebacterium glutamicum ATCC13032
-
-
-

Purification (Commentary)

Purification (Comment) Organism
amylose column chromatography Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cysteamine + NADPH + H+ + O2
-
 Corynebacterium glutamicum ?
-
 ?
cysteamine + NADPH + H+ + O2
-
 Corynebacterium glutamicum ATCC13032 ?
-
 ?
indole + NADPH + H+ + O2
-
 Corynebacterium glutamicum indoxyl + NADP+ + H2O
-
 ?
indole + NADPH + H+ + O2
-
 Corynebacterium glutamicum ATCC13032 indoxyl + NADP+ + H2O
-
 ?
additional information the enzyme does not oxidize glutathione or cysteine Corynebacterium glutamicum ?
-
 ?
additional information the enzyme does not oxidize glutathione or cysteine Corynebacterium glutamicum ATCC13032 ?
-
 ?
thiourea + NADPH + H+ + O2
-
 Corynebacterium glutamicum ?
-
 ?
thiourea + NADPH + H+ + O2
-
 Corynebacterium glutamicum ATCC13032 ?
-
 ?
trimethylamine + NADPH + H+ + O2
-
 Corynebacterium glutamicum ?
-
 ?
trimethylamine + NADPH + H+ + O2
-
 Corynebacterium glutamicum ATCC13032 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Corynebacterium glutamicum

Synonyms

Synonyms Comment Organism
FMO
-
 Corynebacterium glutamicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Corynebacterium glutamicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.67
-
 Thiourea at pH 8.0 and 25°C Corynebacterium glutamicum
1.83
-
 cysteamine at pH 8.0 and 25°C Corynebacterium glutamicum
2.6
-
 trimethylamine at pH 8.0 and 25°C Corynebacterium glutamicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 in 0.1 M potassium phosphate buffer Corynebacterium glutamicum

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Corynebacterium glutamicum
NADPH
-
 Corynebacterium glutamicum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.3
-
 cysteamine at pH 8.0 and 25°C Corynebacterium glutamicum
1.75
-
 Thiourea at pH 8.0 and 25°C Corynebacterium glutamicum
4.5
-
 trimethylamine at pH 8.0 and 25°C Corynebacterium glutamicum