Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.13.8 extracted from

  • Huang, L.; Romero, E.; Ressmann, A.; Rudroff, F.; Hollmann, F.; Fraaije, M.; Kara, S.
    Nicotinamide adenine dinucleotide-dependent redox-neutral convergent cascade for lactonizations with type II flavin-containing monooxygenase (2017), Adv. Synth. Catal., 359, 2142-2148 .
No PubMed abstract available
Search for more literature for 1.14.13.8

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0031
-
 NADPH with bicyclo[3.2.0]hept-2-en-6-one as cosubstrate, at pH 8.0 and 20°C Rhodococcus jostii
0.01
-
 NADH with bicyclo[3.2.0]hept-2-en-6-one as cosubstrate, at pH 8.0 and 20°C Rhodococcus jostii
1
-
 bicyclo[4.2.0]octan-7-one at pH 8.0 and 20°C Rhodococcus jostii
2.1
-
 Cyclobutanone at pH 8.0 and 20°C Rhodococcus jostii

Organism

Organism UniProt Comment Textmining
Rhodococcus jostii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Strep-tag affinity column chromatography Rhodococcus jostii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 bicyclo[4.2.0]octan-7-one + 2 NADH + 2 H+ + 2 O2
-
 Rhodococcus jostii (3aS,7aS)-hexahydro-1-benzofuran-2(3H)-one + (3aR,7aS)-hexahydro-2-benzofuran-1(3H)-one + 2 NAD+ + 2 H2O
-
 ?
bicyclo[3.2.0]hept-2-en-6-one + NADH + H+ + O2
-
 Rhodococcus jostii ?
-
 ?
bicyclo[3.2.0]hept-2-en-6-one + NADPH + H+ + O2
-
 Rhodococcus jostii ?
-
 ?
cyclobutanone + NADH + H+ + O2
-
 Rhodococcus jostii gamma-butyrolactone + NAD+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
FMO-E
-
 Rhodococcus jostii
type II flavin-containing monooxygenase
-
 Rhodococcus jostii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3
-
 Cyclobutanone at pH 8.0 and 20°C Rhodococcus jostii
1.7
-
 bicyclo[4.2.0]octan-7-one at pH 8.0 and 20°C Rhodococcus jostii
2
-
 NADH with bicyclo[3.2.0]hept-2-en-6-one as cosubstrate, at pH 8.0 and 20°C Rhodococcus jostii
2.8
-
 NADPH with bicyclo[3.2.0]hept-2-en-6-one as cosubstrate, at pH 8.0 and 20°C Rhodococcus jostii

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Rhodococcus jostii
NADH the enzyme accepts both NADPH and NADH as cofactor Rhodococcus jostii
NADPH the enzyme accepts both NADPH and NADH as cofactor Rhodococcus jostii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.5
-
 Cyclobutanone at pH 8.0 and 20°C Rhodococcus jostii
0.8
-
 NADH with bicyclo[3.2.0]hept-2-en-6-one as cosubstrate, at pH 8.0 and 20°C Rhodococcus jostii
1
-
 NADPH with bicyclo[3.2.0]hept-2-en-6-one as cosubstrate, at pH 8.0 and 20°C Rhodococcus jostii
1.7
-
 bicyclo[4.2.0]octan-7-one at pH 8.0 and 20°C Rhodococcus jostii