Cloned (Comment) | Organism |
---|---|
expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Schizosaccharomyces pombe |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme in complex with FAD, and NADPH or methimazole, sitting drop vapor diffusion method, purified protein in 10 mM HEPES, pH 7.0, and 150 mM NaCl, versus reservoir solution containing 20% PEG 4000, 0.1 M sodium citrate buffer, pH 5.8, and 1,6-diaminohexane, cryoprotection by 10% v/v glycerol, X-ray diffraction structure determination and analysis at 2.1-2.4 A resolution | Schizosaccharomyces pombe |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methimazole + NADPH + O2 | Schizosaccharomyces pombe | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Schizosaccharomyces pombe | Q9HFE4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme in Escherichia coli strain BL21(DE3) | Schizosaccharomyces pombe |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
N,N-dimethylaniline + NADPH + H+ + O2 = N,N-dimethylaniline N-oxide + NADP+ + H2O | catalytic reaction mechanism via 4alpha-hydroperoxyflavin transient intermediate | Schizosaccharomyces pombe |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Schizosaccharomyces pombe |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methimazole + NADPH + O2 | - |
Schizosaccharomyces pombe | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the active FMO exists in the cell as a complex with a reduced form of the prosthetic group and NADPH cofactor | Schizosaccharomyces pombe |
Synonyms | Comment | Organism |
---|---|---|
FMO | - |
Schizosaccharomyces pombe |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the prosthetic group FAD is an integral part of the protein, the active FMO exists in the cell as a complex with a reduced form of the prosthetic group and NADPH cofactor, binding structure, overview | Schizosaccharomyces pombe | |
NADPH | dependent on, the active FMO exists in the cell as a complex with a reduced form of the prosthetic group and NADPH cofactor, binding structure, overview | Schizosaccharomyces pombe |