BRENDA - Enzyme Database
show all sequences of 1.14.13.79

The gibberellin biosynthetic genes AtKAO1 and AtKAO2 have overlapping roles throughout Arabidopsis development

Regnault, T.; Daviere, J.M.; Heintz, D.; Lange, T.; Achard, P.; Plant J. 80, 462-474 (2014)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene KAO1, phylogenetic analysis and tree
Arabidopsis thaliana
gene KAO2, phylogenetic analysis and tree
Arabidopsis thaliana
Engineering
Protein Variants
Commentary
Organism
additional information
construction of single kao1 and double kao1/kao2 mutants, mutant gibberellin contents and phenotypes, detailed overview
Arabidopsis thaliana
additional information
construction of single kao2 and double kao1/kao2 mutants, mutant gibberellin contents and phenotypes, detailed overview
Arabidopsis thaliana
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Arabidopsis thaliana
5783
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ent-kaur-16-en-19-oate + 3 NADPH + 3 H+ + 3 O2
Arabidopsis thaliana
overall reaction
gibberellin A12 + 3 NADP+ + 4 H2O
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Arabidopsis thaliana
O23051
-
-
Arabidopsis thaliana
Q9C5Y2
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cotyledon
-
Arabidopsis thaliana
-
hypocotyl
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
additional information
expression patterns of isozymes reveal that both genes, KAO1 and KAO2, are mainly expressed in germinating seeds and young developing organs, thus suggesting functional redundancy. The expression of KAO2 is much stronger than that of KAO1. KAO1 expression is almost exclusively restricted to the shoot apical meristem, the leaf primordia and the root meristem
Arabidopsis thaliana
-
additional information
expression patterns of isozymes reveal that both genes, KAO1 and KAO2, are mainly expressed in germinating seeds and young developing organs, thus suggesting functional redundancy. The expression of KAO2 is much stronger than that of KAO1. KAO2 is expressed in hypocotyl and petioles of the cotyledons, in the vegetative shoot apical meristem and leaf primordia, and in the meristematic zone of the root and lateral roots
Arabidopsis thaliana
-
petiole
-
Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
seed
germinating
Arabidopsis thaliana
-
seedling
-
Arabidopsis thaliana
-
shoot
-
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ent-kaur-16-en-19-oate + 3 NADPH + 3 H+ + 3 O2
overall reaction
746078
Arabidopsis thaliana
gibberellin A12 + 3 NADP+ + 4 H2O
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
KAO
-
Arabidopsis thaliana
KAO1
-
Arabidopsis thaliana
KAO2
-
Arabidopsis thaliana
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Arabidopsis thaliana
NADPH
-
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
gene KAO1, phylogenetic analysis and tree
Arabidopsis thaliana
gene KAO2, phylogenetic analysis and tree
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Arabidopsis thaliana
NADPH
-
Arabidopsis thaliana
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
construction of single kao1 and double kao1/kao2 mutants, mutant gibberellin contents and phenotypes, detailed overview
Arabidopsis thaliana
additional information
construction of single kao2 and double kao1/kao2 mutants, mutant gibberellin contents and phenotypes, detailed overview
Arabidopsis thaliana
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Arabidopsis thaliana
5783
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
ent-kaur-16-en-19-oate + 3 NADPH + 3 H+ + 3 O2
Arabidopsis thaliana
overall reaction
gibberellin A12 + 3 NADP+ + 4 H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cotyledon
-
Arabidopsis thaliana
-
hypocotyl
-
Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
additional information
expression patterns of isozymes reveal that both genes, KAO1 and KAO2, are mainly expressed in germinating seeds and young developing organs, thus suggesting functional redundancy. The expression of KAO2 is much stronger than that of KAO1. KAO1 expression is almost exclusively restricted to the shoot apical meristem, the leaf primordia and the root meristem
Arabidopsis thaliana
-
additional information
expression patterns of isozymes reveal that both genes, KAO1 and KAO2, are mainly expressed in germinating seeds and young developing organs, thus suggesting functional redundancy. The expression of KAO2 is much stronger than that of KAO1. KAO2 is expressed in hypocotyl and petioles of the cotyledons, in the vegetative shoot apical meristem and leaf primordia, and in the meristematic zone of the root and lateral roots
Arabidopsis thaliana
-
petiole
-
Arabidopsis thaliana
-
root
-
Arabidopsis thaliana
-
seed
germinating
Arabidopsis thaliana
-
seedling
-
Arabidopsis thaliana
-
shoot
-
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ent-kaur-16-en-19-oate + 3 NADPH + 3 H+ + 3 O2
overall reaction
746078
Arabidopsis thaliana
gibberellin A12 + 3 NADP+ + 4 H2O
-
-
-
?
General Information
General Information
Commentary
Organism
evolution
ent-kaurenoic acid oxidase (KAO) belongs to a class of cytochrome P450 monooxygenases of the subfamily CYP88A
Arabidopsis thaliana
malfunction
kao1 and kao2 single mutants are indistinguishable from wild-type plants. By contrast, the kao1/kao2 double mutant exhibits typical non-germinating GA-dwarf phenotypes, similar to those observed in the severely gibberellic acid-deficient ga1-3 mutant. Phenotype, overview. Loss of KAO1 and KAO2 function affects seed germination and alters plant growth and reproductive development. Effects of the kao mutations on GA signaling and on feedback regulation of GA metabolism gene expression
Arabidopsis thaliana
metabolism
the enzyme is important in the gibberellin biosynthesis, pathway overview
Arabidopsis thaliana
physiological function
ent-kaurenoic acid oxidase (KAO) catalyzes the conversion of ent-kaurenoic acid (KA) to gibberellin (GA) GA12, the precursor of all gibberellins, thereby playing an important role in determining gibberellin concentration in plants, overlapping role of KAO1 and KAO2 throughout Arabidopsis development, overview
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
evolution
ent-kaurenoic acid oxidase (KAO) belongs to a class of cytochrome P450 monooxygenases of the subfamily CYP88A
Arabidopsis thaliana
malfunction
kao1 and kao2 single mutants are indistinguishable from wild-type plants. By contrast, the kao1/kao2 double mutant exhibits typical non-germinating GA-dwarf phenotypes, similar to those observed in the severely gibberellic acid-deficient ga1-3 mutant. Phenotype, overview. Loss of KAO1 and KAO2 function affects seed germination and alters plant growth and reproductive development. Effects of the kao mutations on GA signaling and on feedback regulation of GA metabolism gene expression
Arabidopsis thaliana
metabolism
the enzyme is important in the gibberellin biosynthesis, pathway overview
Arabidopsis thaliana
physiological function
ent-kaurenoic acid oxidase (KAO) catalyzes the conversion of ent-kaurenoic acid (KA) to gibberellin (GA) GA12, the precursor of all gibberellins, thereby playing an important role in determining gibberellin concentration in plants, overlapping role of KAO1 and KAO2 throughout Arabidopsis development, overview
Arabidopsis thaliana
Other publictions for EC 1.14.13.79
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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The gibberellin biosynthetic ...
Arabidopsis thaliana
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2014
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2
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2
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2
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2
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2
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15
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2
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4
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4
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2
4
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2
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2
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2
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15
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2
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8
8
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