BRENDA - Enzyme Database
show all sequences of 1.14.13.70

Azole antifungal sensitivity of sterol 14alpha-demethylase (CYP51) and CYP5218 from Malassezia globosa

Warrilow, A.G.; Price, C.L.; Parker, J.E.; Rolley, N.J.; Smyrniotis, C.J.; Hughes, D.D.; Thoss, V.; Nes, W.D.; Kelly, D.E.; Holman, T.R.; Kelly, S.L.; Sci. Rep. 6, 27690 (2016)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
-
Malassezia globosa
Inhibitors
Inhibitors
Commentary
Organism
Structure
fluconazole
-
Malassezia globosa
itraconazole
-
Malassezia globosa
ketaminazole
-
Malassezia globosa
ketoconazole
-
Malassezia globosa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0421
-
obtusifoliol
37°C, pH 7.2
Malassezia globosa
0.0551
-
lanosterol
37°C, pH 7.2
Malassezia globosa
0.0559
-
eburicol
37°C, pH 7.2
Malassezia globosa
Organism
Organism
UniProt
Commentary
Textmining
Malassezia globosa
A8Q3I7
-
-
Malassezia globosa CBS 7966
A8Q3I7
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Malassezia globosa
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
eburicol + NADPH + H+ + O2
-
746454
Malassezia globosa
?
-
-
-
?
eburicol + NADPH + H+ + O2
-
746454
Malassezia globosa CBS 7966
?
-
-
-
?
lanosterol + NADPH + H+ + O2
-
746454
Malassezia globosa
?
-
-
-
?
lanosterol + NADPH + H+ + O2
-
746454
Malassezia globosa CBS 7966
?
-
-
-
?
obtusifoliol + NADPH + H+ + O2
-
746454
Malassezia globosa
?
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
CYP51
-
Malassezia globosa
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Malassezia globosa
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.03
-
lanosterol
37°C, PH 7.2
Malassezia globosa
0.06
-
obtusifoliol
37°C, PH 7.2
Malassezia globosa
0.09
-
eburicol
37°C, PH 7.2
Malassezia globosa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at
Malassezia globosa
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.176
-
37°C, pH 7.2
Malassezia globosa
ketoconazole
0.188
-
37°C, pH 7.2
Malassezia globosa
itraconazole
0.206
-
37°C, pH 7.2
Malassezia globosa
fluconazole
0.321
-
37°C, pH 7.2
Malassezia globosa
ketaminazole
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Malassezia globosa
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.176
-
37°C, pH 7.2
Malassezia globosa
ketoconazole
0.188
-
37°C, pH 7.2
Malassezia globosa
itraconazole
0.206
-
37°C, pH 7.2
Malassezia globosa
fluconazole
0.321
-
37°C, pH 7.2
Malassezia globosa
ketaminazole
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
fluconazole
-
Malassezia globosa
itraconazole
-
Malassezia globosa
ketaminazole
-
Malassezia globosa
ketoconazole
-
Malassezia globosa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0421
-
obtusifoliol
37°C, pH 7.2
Malassezia globosa
0.0551
-
lanosterol
37°C, pH 7.2
Malassezia globosa
0.0559
-
eburicol
37°C, pH 7.2
Malassezia globosa
Purification (Commentary) (protein specific)
Commentary
Organism
-
Malassezia globosa
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
eburicol + NADPH + H+ + O2
-
746454
Malassezia globosa
?
-
-
-
?
eburicol + NADPH + H+ + O2
-
746454
Malassezia globosa CBS 7966
?
-
-
-
?
lanosterol + NADPH + H+ + O2
-
746454
Malassezia globosa
?
-
-
-
?
lanosterol + NADPH + H+ + O2
-
746454
Malassezia globosa CBS 7966
?
-
-
-
?
obtusifoliol + NADPH + H+ + O2
-
746454
Malassezia globosa
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Malassezia globosa
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.03
-
lanosterol
37°C, PH 7.2
Malassezia globosa
0.06
-
obtusifoliol
37°C, PH 7.2
Malassezia globosa
0.09
-
eburicol
37°C, PH 7.2
Malassezia globosa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at
Malassezia globosa
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.54
-
lanosterol
37°C, pH 7.2
Malassezia globosa
1.4
-
obtusifoliol
37°C, pH 7.2
Malassezia globosa
1.6
-
eburicol
37°C, pH 7.2
Malassezia globosa
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.54
-
lanosterol
37°C, pH 7.2
Malassezia globosa
1.4
-
obtusifoliol
37°C, pH 7.2
Malassezia globosa
1.6
-
eburicol
37°C, pH 7.2
Malassezia globosa
Other publictions for EC 1.14.13.70
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744274
Park
Nitric oxide stimulates cellu ...
Homo sapiens
Biochem. J.
474
3241-3252
2017
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1
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1
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1
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1
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1
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1
1
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744843
Emami
An overview of azoles targeti ...
Leishmania major, Leishmania mexicana, Leishmania infantum
Eur. J. Med. Chem.
135
241-259
2017
-
6
-
-
-
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39
-
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3
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6
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6
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4
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6
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4
39
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6
-
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6
-
-
-
-
-
-
-
-
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6
6
-
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-
744860
Borcea
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Synthesis and molecular docki ...
Candida albicans SC5314
Farmacia
65
683-689
2017
-
-
-
-
-
-
10
-
-
-
-
-
-
1
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-
-
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10
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-
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-
745117
Stana
Design, synthesis and antifun ...
Candida albicans SC5314
Int. J. Mol. Sci.
18
E177
2017
-
2
-
-
-
-
6
-
-
-
-
-
-
1
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2
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2
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6
-
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-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
745378
Hargrove
Structural analyses of Candid ...
Candida albicans
J. Biol. Chem.
292
6728-6743
2017
-
-
1
1
-
-
8
1
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1
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1
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1
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1
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1
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1
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1
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8
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1
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1
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1
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1
-
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-
-
-
-
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1
1
745682
Alvarez-Rueda
The amino acid substitution N ...
Candida albicans
Med. Mycol.
54
764-775
2016
-
-
1
-
1
-
1
-
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1
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1
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1
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1
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1
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1
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1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
746216
Tyndall
Structural and functional elu ...
Saccharomyces cerevisiae, Saccharomyces cerevisiae YJM789
PLoS ONE
11
e0167485
2016
-
-
1
1
2
-
7
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
3
-
-
-
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-
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-
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-
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1
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1
2
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7
-
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-
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1
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
746454
Warrilow
Azole antifungal sensitivity ...
Malassezia globosa, Malassezia globosa CBS 7966
Sci. Rep.
6
27690
2016
-
-
1
-
-
-
4
3
-
-
-
-
-
2
-
-
1
-
-
-
-
-
5
-
1
1
-
-
3
1
-
-
-
-
-
4
-
-
1
-
-
-
-
4
4
-
3
-
-
-
-
-
-
-
1
-
-
-
-
5
-
1
-
-
3
1
-
-
-
-
-
-
-
3
3
744385
Morrison
Identification, modeling and ...
Danio rerio
Biochim. Biophys. Acta
1840
1825-1836
2014
-
-
1
-
-
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2
-
-
-
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1
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-
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1
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1
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3
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1
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2
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1
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1
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-
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-
-
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-
745522
Friggeri
Structural basis for rational ...
Trypanosoma cruzi, Trypanosoma cruzi CL Brener
J. Med. Chem.
57
6704-6717
2014
-
2
1
1
-
-
2
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
2
-
1
-
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-
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-
-
-
-
-
-
-
2
1
-
1
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-
-
2
-
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1
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2
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