Cloned (Comment) | Organism |
---|---|
the enzyme is encoded in the ph operon, expression in Escherichia coli strain JM109 | Pseudomonas stutzeri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics and regioselectivity, overview | Pseudomonas stutzeri | |
0.0006 | - |
phenol | 25°C, recombinant enzyme | Pseudomonas stutzeri | |
0.0006 | - |
4-Cresol | 25°C, recombinant enzyme | Pseudomonas stutzeri | |
0.0018 | - |
3-cresol | 25°C, recombinant enzyme | Pseudomonas stutzeri | |
0.0031 | - |
2-Cresol | 25°C, recombinant enzyme | Pseudomonas stutzeri | |
0.022 | - |
Toluene | 25°C, recombinant enzyme | Pseudomonas stutzeri | |
0.025 | - |
Benzene | 25°C, recombinant enzyme | Pseudomonas stutzeri | |
0.0325 | - |
2-xylene | 25°C, recombinant enzyme | Pseudomonas stutzeri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas stutzeri | - |
- |
- |
Pseudomonas stutzeri OX1 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 2-cresol + NADPH + O2 | - |
Pseudomonas stutzeri | 3-methylcatechol + 4-methylcatechol + NADP+ + H2O | - |
? | |
2 2-xylene + 2 NADPH + 2 H+ + 2 O2 | - |
Pseudomonas stutzeri | 2,3-dimethylphenol + 3,4-dimethylphenol + 2 NADP+ + 2 H2O | - |
? | |
2 2-xylene + 2 NADPH + 2 H+ + 2 O2 | - |
Pseudomonas stutzeri OX1 | 2,3-dimethylphenol + 3,4-dimethylphenol + 2 NADP+ + 2 H2O | - |
? | |
3 toluene + 3 NADPH + 3 H+ + 3 O2 | regioselectivity, overview | Pseudomonas stutzeri | 2-cresol + 3-cresol + 4-cresol + 3 NADP+ + 3 H2O | - |
? | |
3 toluene + 3 NADPH + 3 H+ + 3 O2 | regioselectivity, overview | Pseudomonas stutzeri OX1 | 2-cresol + 3-cresol + 4-cresol + 3 NADP+ + 3 H2O | - |
? | |
3-cresol + NADPH + O2 | - |
Pseudomonas stutzeri | 3-methylcatechol + 4-methylcatechol + NADP+ + H2O | - |
? | |
4-cresol + NADPH + O2 | best substrate | Pseudomonas stutzeri | 4-methylcatechol + NADP+ + H2O | - |
? | |
benzene + NADPH + O2 + H+ | - |
Pseudomonas stutzeri | phenol + NADP+ + H2O | - |
? | |
benzene + NADPH + O2 + H+ | - |
Pseudomonas stutzeri OX1 | phenol + NADP+ + H2O | - |
? | |
additional information | the enzyme hydroxylates benzenes to catechols via the intermediate production of phenols | Pseudomonas stutzeri | ? | - |
? | |
additional information | the enzyme hydroxylates benzenes to catechols via the intermediate production of phenols | Pseudomonas stutzeri OX1 | ? | - |
? | |
phenol + NADPH + O2 | - |
Pseudomonas stutzeri | catechol + NADP+ + H2O | - |
? | |
phenol + NADPH + O2 | - |
Pseudomonas stutzeri OX1 | catechol + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the enzyme is a multicomponent monooxygenase | Pseudomonas stutzeri |
Synonyms | Comment | Organism |
---|---|---|
phenol hydroxylase | - |
Pseudomonas stutzeri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas stutzeri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
Benzene | 25°C, recombinant enzyme | Pseudomonas stutzeri | |
0.125 | - |
2-xylene | 25°C, recombinant enzyme | Pseudomonas stutzeri | |
0.15 | - |
Toluene | 25°C, recombinant enzyme | Pseudomonas stutzeri | |
0.61 | - |
3-cresol | 25°C, recombinant enzyme | Pseudomonas stutzeri | |
0.77 | - |
4-Cresol | 25°C, recombinant enzyme | Pseudomonas stutzeri | |
0.95 | - |
2-Cresol | 25°C, recombinant enzyme | Pseudomonas stutzeri | |
1.02 | - |
phenol | 25°C, recombinant enzyme | Pseudomonas stutzeri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Pseudomonas stutzeri |