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Literature summary for 1.14.13.69 extracted from

  • Perry, A.; Smith, T.J.
    Protocol for mutagenesis of alkene monooxygenase and screening for modified enantiocomposition of the epoxypropane product (2006), J. Biomol. Screen., 11, 553-556.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes Rhodococcus rhodochrous

Protein Variants

Protein Variants Comment Organism
additional information quasi random mutation of the large subunit of the epoxygenase component, AmoC alters the enantioselectivity of the enzyme, screening for altered epoxidation abilities Rhodococcus rhodochrous

Metals/Ions

Metals/Ions Comment Organism Structure
Iron the enzyme possesses a dinuclear iron center within the large subunit of the epoxygenase component, AmoC, mutagenesis of AmoC alters the enantioselectivity of the enzyme Rhodococcus rhodochrous

Organism

Organism UniProt Comment Textmining
Rhodococcus rhodochrous
-
-
-
Rhodococcus rhodochrous B-276
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is a three-component system encoded by the 4-gene operon amoABCD, which catalyzes the stereoselective epoxidation of aliphatic alkenes yielding primarily the R enantiomer Rhodococcus rhodochrous ?
-
?
additional information the enzyme is a three-component system encoded by the 4-gene operon amoABCD, which catalyzes the stereoselective epoxidation of aliphatic alkenes yielding primarily the R enantiomer Rhodococcus rhodochrous B-276 ?
-
?
propene + NADH + H+ + O2
-
Rhodococcus rhodochrous 1,2-epoxypropane + NAD+ + H2O
-
?
propene + NADH + H+ + O2
-
Rhodococcus rhodochrous B-276 1,2-epoxypropane + NAD+ + H2O
-
?

Synonyms

Synonyms Comment Organism
AMO
-
Rhodococcus rhodochrous

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Rhodococcus rhodochrous

Cofactor

Cofactor Comment Organism Structure
NADH
-
Rhodococcus rhodochrous