Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | phenylacetate and its hydroxyderivatives strongly induce the enzyme | Aspergillus nidulans |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression pattern, sequence comparison, expression in a recombinant Aspergillus nidulans strain | Aspergillus nidulans |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a phacB-disrupted strain DELTAphacB does not grow on 3-hydroxy-, 4-hydroxy-, or 3,4-dihydroxy-phenylacetate, overview | Aspergillus nidulans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the enzyme is a cytochrome P450 monooxygenase containing a heme iron | Aspergillus nidulans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxyphenylacetate + NAD(P)H + O2 | Aspergillus nidulans | low activity, step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview | 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O | - |
? | |
2-hydroxyphenylacetate + NAD(P)H + O2 | Aspergillus nidulans BiA1 | low activity, step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview | 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O | - |
? | |
3,4-dihydroxyphenylacetate + NAD(P)H + O2 | Aspergillus nidulans | step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview | 2,4,5-trihydroxyphenylacetate + NAD(P)+ + H2O | the product is a substrate for homogentisate dioxygenase | ? | |
3,4-dihydroxyphenylacetate + NAD(P)H + O2 | Aspergillus nidulans BiA1 | step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview | 2,4,5-trihydroxyphenylacetate + NAD(P)+ + H2O | the product is a substrate for homogentisate dioxygenase | ? | |
3-hydroxyphenylacetate + NAD(P)H + O2 | Aspergillus nidulans | step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview | 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O | the product is a substrate for homogentisate dioxygenase | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus nidulans | Q078T0 | gene phacB | - |
Aspergillus nidulans BiA1 | Q078T0 | gene phacB | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Aspergillus nidulans | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxyphenylacetate + NAD(P)H + O2 | low activity | Aspergillus nidulans | 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O | - |
? | |
2-hydroxyphenylacetate + NAD(P)H + O2 | low activity, step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview | Aspergillus nidulans | 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O | - |
? | |
2-hydroxyphenylacetate + NAD(P)H + O2 | low activity | Aspergillus nidulans BiA1 | 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O | - |
? | |
2-hydroxyphenylacetate + NAD(P)H + O2 | low activity, step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview | Aspergillus nidulans BiA1 | 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O | - |
? | |
3,4-dihydroxyphenylacetate + NAD(P)H + O2 | - |
Aspergillus nidulans | 2,4,5-trihydroxyphenylacetate + NAD(P)+ + H2O | - |
? | |
3,4-dihydroxyphenylacetate + NAD(P)H + O2 | step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview | Aspergillus nidulans | 2,4,5-trihydroxyphenylacetate + NAD(P)+ + H2O | the product is a substrate for homogentisate dioxygenase | ? | |
3,4-dihydroxyphenylacetate + NAD(P)H + O2 | - |
Aspergillus nidulans BiA1 | 2,4,5-trihydroxyphenylacetate + NAD(P)+ + H2O | - |
? | |
3,4-dihydroxyphenylacetate + NAD(P)H + O2 | step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview | Aspergillus nidulans BiA1 | 2,4,5-trihydroxyphenylacetate + NAD(P)+ + H2O | the product is a substrate for homogentisate dioxygenase | ? | |
3-hydroxyphenylacetate + NAD(P)H + O2 | - |
Aspergillus nidulans | 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O | - |
? | |
3-hydroxyphenylacetate + NAD(P)H + O2 | step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview | Aspergillus nidulans | 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O | the product is a substrate for homogentisate dioxygenase | ? | |
3-hydroxyphenylacetate + NAD(P)H + O2 | - |
Aspergillus nidulans BiA1 | 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3,4-dihydroxyphenylacetate 6-hydroxylase | - |
Aspergillus nidulans |
CYP504B | - |
Aspergillus nidulans |
More | the enzyme is a member of a the CYP450 subfamily | Aspergillus nidulans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Aspergillus nidulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Aspergillus nidulans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Aspergillus nidulans | |
NADPH | - |
Aspergillus nidulans |