Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.13.63 extracted from

  • Ferrer-Sevillano, F.; Fernandez-Canon, J.M.
    Novel phacB-encoded cytochrome P450 monooxygenase from Aspergillus nidulans with 3-hydroxyphenylacetate 6-hydroxylase and 3,4-dihydroxyphenylacetate 6-hydroxylase activities (2007), Eukaryot. Cell, 6, 514-520.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information phenylacetate and its hydroxyderivatives strongly induce the enzyme Aspergillus nidulans

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression pattern, sequence comparison, expression in a recombinant Aspergillus nidulans strain Aspergillus nidulans

Protein Variants

Protein Variants Comment Organism
additional information a phacB-disrupted strain DELTAphacB does not grow on 3-hydroxy-, 4-hydroxy-, or 3,4-dihydroxy-phenylacetate, overview Aspergillus nidulans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the enzyme is a cytochrome P450 monooxygenase containing a heme iron Aspergillus nidulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-hydroxyphenylacetate + NAD(P)H + O2 Aspergillus nidulans low activity, step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O
-
?
2-hydroxyphenylacetate + NAD(P)H + O2 Aspergillus nidulans BiA1 low activity, step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O
-
?
3,4-dihydroxyphenylacetate + NAD(P)H + O2 Aspergillus nidulans step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview 2,4,5-trihydroxyphenylacetate + NAD(P)+ + H2O the product is a substrate for homogentisate dioxygenase ?
3,4-dihydroxyphenylacetate + NAD(P)H + O2 Aspergillus nidulans BiA1 step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview 2,4,5-trihydroxyphenylacetate + NAD(P)+ + H2O the product is a substrate for homogentisate dioxygenase ?
3-hydroxyphenylacetate + NAD(P)H + O2 Aspergillus nidulans step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O the product is a substrate for homogentisate dioxygenase ?

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans Q078T0 gene phacB
-
Aspergillus nidulans BiA1 Q078T0 gene phacB
-

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Aspergillus nidulans
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxyphenylacetate + NAD(P)H + O2 low activity Aspergillus nidulans 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O
-
?
2-hydroxyphenylacetate + NAD(P)H + O2 low activity, step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview Aspergillus nidulans 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O
-
?
2-hydroxyphenylacetate + NAD(P)H + O2 low activity Aspergillus nidulans BiA1 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O
-
?
2-hydroxyphenylacetate + NAD(P)H + O2 low activity, step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview Aspergillus nidulans BiA1 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O
-
?
3,4-dihydroxyphenylacetate + NAD(P)H + O2
-
Aspergillus nidulans 2,4,5-trihydroxyphenylacetate + NAD(P)+ + H2O
-
?
3,4-dihydroxyphenylacetate + NAD(P)H + O2 step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview Aspergillus nidulans 2,4,5-trihydroxyphenylacetate + NAD(P)+ + H2O the product is a substrate for homogentisate dioxygenase ?
3,4-dihydroxyphenylacetate + NAD(P)H + O2
-
Aspergillus nidulans BiA1 2,4,5-trihydroxyphenylacetate + NAD(P)+ + H2O
-
?
3,4-dihydroxyphenylacetate + NAD(P)H + O2 step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview Aspergillus nidulans BiA1 2,4,5-trihydroxyphenylacetate + NAD(P)+ + H2O the product is a substrate for homogentisate dioxygenase ?
3-hydroxyphenylacetate + NAD(P)H + O2
-
Aspergillus nidulans 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O
-
?
3-hydroxyphenylacetate + NAD(P)H + O2 step in the 2,5-dihydroxyphenylacetate, i.e.homogentisic acid, catabolic pathway, overview Aspergillus nidulans 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O the product is a substrate for homogentisate dioxygenase ?
3-hydroxyphenylacetate + NAD(P)H + O2
-
Aspergillus nidulans BiA1 2,5-dihydroxyphenylacetate + NAD(P)+ + H2O
-
?

Synonyms

Synonyms Comment Organism
3,4-dihydroxyphenylacetate 6-hydroxylase
-
Aspergillus nidulans
CYP504B
-
Aspergillus nidulans
More the enzyme is a member of a the CYP450 subfamily Aspergillus nidulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Aspergillus nidulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Aspergillus nidulans

Cofactor

Cofactor Comment Organism Structure
NADH
-
Aspergillus nidulans
NADPH
-
Aspergillus nidulans