Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetone | 15%, 1.2fold activation. Strong decrease in activity above 15% | Pseudomonas putida | |
ethanol | 15%, 1.7fold activation. Strong decrease in activity above 15% | Pseudomonas putida |
General Stability | Organism |
---|---|
50% loss of enzyme activity after 96 h without additions | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | partial inhibition | Pseudomonas putida | |
Cu2+ | - |
Pseudomonas putida | |
H2O2 | - |
Pseudomonas putida | |
Hg2+ | - |
Pseudomonas putida | |
iodoacetate | partial inhibition | Pseudomonas putida | |
Sodium dithionite | - |
Pseudomonas putida | |
Zn2+ | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
1H-4-Oxoquinoline | - |
Pseudomonas putida | |
0.087 | - |
NADH | - |
Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
3 * 42000, SDS-PAGE | Pseudomonas putida |
126000 | - |
gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas putida | inducible by 1H-4-oxoquinoline | ? | - |
? | |
additional information | Pseudomonas putida 33/1 | inducible by 1H-4-oxoquinoline | ? | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | 40% loss of activity after 2 h in 15% acetone | Pseudomonas putida |
Ethanol | stable for 2 h in 15% ethanol, 50% loss of activity after 24 h | Pseudomonas putida |
Ethylene glycol | completely stable after 96 h in 50% ethylene glycol | Pseudomonas putida |
additional information | 50% loss of enzyme activity after 96 h without addition of organic solvents | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida 33/1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.8 | - |
- |
Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1H-4-Oxoquinoline + NADH + O2 | - |
Pseudomonas putida | 1H-3-Hydroxy-4-oxoquinoline + NAD+ + H2O | - |
? | |
1H-4-Oxoquinoline + NADH + O2 | - |
Pseudomonas putida 33/1 | 1H-3-Hydroxy-4-oxoquinoline + NAD+ + H2O | - |
? | |
1H-4-Oxoquinoline + NADPH + O2 | 50% of the activity with NADH | Pseudomonas putida | 1H-3-Hydroxy-4-oxoquinoline + NADP+ + H2O | - |
? | |
1H-4-Oxoquinoline + NADPH + O2 | 50% of the activity with NADH | Pseudomonas putida 33/1 | 1H-3-Hydroxy-4-oxoquinoline + NADP+ + H2O | - |
? | |
additional information | inducible by 1H-4-oxoquinoline | Pseudomonas putida | ? | - |
? | |
additional information | inducible by 1H-4-oxoquinoline | Pseudomonas putida 33/1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 3 * 42000, SDS-PAGE | Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Pseudomonas putida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
strong inactivation above | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | required as cofactor | Pseudomonas putida | |
NADPH | 50% of the activity compared to NADH | Pseudomonas putida |