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Literature summary for 1.14.13.54 extracted from

  • Itagaki, E.
    Studies on steroid monooxygenase from Cylindrocarpon radicicola ATCC 11011. Purification and characterization (1986), J. Biochem., 99, 815-824.
    View publication on PubMed
Search for more literature for 1.14.13.54

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
 progesterone
-
 Ilyonectria destructans
0.0043
-
 NADPH with progesterone and O2 as cosubstrates Ilyonectria destructans
0.07
-
 O2 with progesterone and NADPH as cosubstrates Ilyonectria destructans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 2 * 56000, SDS-PAGE Ilyonectria destructans
116000
-
 gel filtration, gel electrophoresis Ilyonectria destructans

Organism

Organism UniProt Comment Textmining
Ilyonectria destructans
-
 ATTC 11011
-
Ilyonectria destructans ATTC 11011
-
 ATTC 11011
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ilyonectria destructans

Source Tissue

Source Tissue Comment Organism Textmining

Storage Stability

Storage Stability Organism
-80°C, Tris buffer, stable for more than 6 months, enzyme in crude extract Ilyonectria destructans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-Pregnadiene-17alpha,21-diol-3,11,20-trione + NADPH + O2
-
 Ilyonectria destructans ?
-
 ?
11beta-Hydroxyprogesterone + NADPH + O2
-
 Ilyonectria destructans ?
-
 ?
11beta-Hydroxyprogesterone + NADPH + O2
-
 Ilyonectria destructans ATTC 11011 ?
-
 ?
17alpha-Hydroxyprogesterone + NADPH + O2
-
 Ilyonectria destructans androstenedione + acetate + NADP+ + H2O
-
 ?
17alpha-Hydroxyprogesterone + NADPH + O2
-
 Ilyonectria destructans ATTC 11011 androstenedione + acetate + NADP+ + H2O
-
 ?
18-Hydroxydeoxycorticosterone + NADPH + O2
-
 Ilyonectria destructans ?
-
 ?
21-Deoxycortisol + NADPH + O2
-
 Ilyonectria destructans ?
-
 ?
Aldosterone + NADPH + O2
-
 Ilyonectria destructans ?
-
 ?
androstenedione + NADPH + O2
-
 Ilyonectria destructans 3-oxo-13,17-secoandrost-4-eno-17,13-alpha-lactone + NADP+ + H2O
-
 ?
androstenedione + NADPH + O2
-
 Ilyonectria destructans ATTC 11011 3-oxo-13,17-secoandrost-4-eno-17,13-alpha-lactone + NADP+ + H2O
-
 ?
Corticosterone + NADPH + O2
-
 Ilyonectria destructans ?
-
 ?
Cortisol + NADPH + O2
-
 Ilyonectria destructans ?
-
 ?
Cortisone + NADPH + O2
-
 Ilyonectria destructans ?
-
 ?
deoxycorticosterone + NADPH + O2
-
 Ilyonectria destructans ?
-
 ?
deoxycorticosterone + NADPH + O2
-
 Ilyonectria destructans ATTC 11011 ?
-
 ?
progesterone + NADPH + H+ + O2
-
 Ilyonectria destructans Testosterone acetate + NADP+ + H2O
-
 ?
progesterone + NADPH + H+ + O2
-
 Ilyonectria destructans ATTC 11011 Testosterone acetate + NADP+ + H2O
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 56000, SDS-PAGE Ilyonectria destructans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 progesterone monooxygenase reaction Ilyonectria destructans

pH Range

pH Minimum pH Maximum Comment Organism
6 9 pH 6.0: about 35% of maximal activity, pH 9.0: about 65% of maximal activity, progesterone monooxygenase activity Ilyonectria destructans

Cofactor

Cofactor Comment Organism Structure
FAD enzyme contains 1.6 mol of FAD per mol of enzyme Ilyonectria destructans