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Literature summary for 1.14.13.50 extracted from

  • Aregbesola, O.A.; Kumar, A.; Mokoena, M.P.; Olaniran, A.O.
    Whole-genome sequencing, genome mining, metabolic reconstruction and evolution of pentachlorophenol and other xenobiotic degradation pathways in Bacillus tropicus strain AOA-CPS1 (2021), Funct. Integr. Genomics, 21, 171-193 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
analogues of PCP catabolic genes (cpsABCDE, and p450) are successfully amplified from the whole genome of BtAOA using the primers designed based on the gene sequences retrieved from the whole genome annotation data submitted at NCBI Bacillus tropicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
Pentachlorophenol recombinant CpsB, pH 7.0, 30°C Bacillus tropicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pentachlorophenol + NADH + H+ + O2 Bacillus tropicus
-
2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O
-
?
pentachlorophenol + NADH + H+ + O2 Bacillus tropicus AOA-CPS1
-
2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O
-
?

Organism

Organism UniProt Comment Textmining
Bacillus tropicus
-
-
-
Bacillus tropicus AOA-CPS1
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pentachlorophenol + NADH + H+ + O2
-
Bacillus tropicus 2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O
-
?
pentachlorophenol + NADH + H+ + O2
-
Bacillus tropicus AOA-CPS1 2,3,5,6-tetrachloro-1,4-benzoquinone + NADP+ + chloride + H2O
-
?

Subunits

Subunits Comment Organism
? x * 64200, about, SDS-PAGE Bacillus tropicus

Synonyms

Synonyms Comment Organism
CpsB
-
Bacillus tropicus
GM610_18120 locus name Bacillus tropicus
PCP 4-monooxygenase
-
Bacillus tropicus
PCP-4-monooxygenase
-
Bacillus tropicus
PcpB
-
Bacillus tropicus
Phe4MO
-
Bacillus tropicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Bacillus tropicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 30 stable at Bacillus tropicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.58
-
Pentachlorophenol recombinant CpsB, pH 7.0, 30°C Bacillus tropicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Bacillus tropicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7.5 stable at Bacillus tropicus

Cofactor

Cofactor Comment Organism Structure
NADH
-
Bacillus tropicus

pI Value

Organism Comment pI Value Maximum pI Value
Bacillus tropicus sequence calculation
-
5.84

General Information

General Information Comment Organism
evolution evolution of PCP catabolic genes in BtAOA, overview Bacillus tropicus
metabolism two pathways are proposed for pentachlorophenol (PCP) transformation in BtAOA. In the first pathway, 2,6-bis(1,1-dimethylethyl)phenol, 1-methoxy-5-trimetylsilyoxyhexane, trimethylsilyl 2-butoxyacetate and methyl 2-hydroxyl-3-methylbutanoate fragments are found. The metabolites detected for the proposed second pathway include 1,3-dimethyl-4,6-diisopropylbenzene, 2,4-dimethylbenzenecarboxaldehyde and 2,5-dimethylbenzaldehyde. Both 2,6-di-tert-butylbenzoquinone and 1,3-dimethyl-4,6-diisopropylbenzene cannot be metabolites from the same pathway, it could be that, one of the pathways is initiated by PcpB and the other by cytochrome P450 monooxygenase. Metabolic reconstruction of BtAOA xenobiotics biodegradation pathways, pathway regulation analysis, overview Bacillus tropicus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
26.94
-
Pentachlorophenol recombinant CpsB, pH 7.0, 30°C Bacillus tropicus