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Literature summary for 1.14.13.50 extracted from

  • Nakamura, T.; Motoyama, T.; Hirono, S.; Yamaguchi, I.
    Identification, characterization, and site-directed mutagenesis of recombinant pentachlorophenol 4-monooxygenase (2004), Biochim. Biophys. Acta, 1700, 151-159.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression of His-tagged wild-type and mutant enzymes in Escherichia coli Sphingobium chlorophenolicum

Protein Variants

Protein Variants Comment Organism
F85A site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme Sphingobium chlorophenolicum
R235A site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme Sphingobium chlorophenolicum
R235E site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme Sphingobium chlorophenolicum
R235K site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme Sphingobium chlorophenolicum
Y216A site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme Sphingobium chlorophenolicum
Y397A site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme Sphingobium chlorophenolicum
Y397F site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme Sphingobium chlorophenolicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Sphingobium chlorophenolicum
0.0005
-
Pentachlorophenol pH 7.0, 25°C, recombinant wild-type enzyme Sphingobium chlorophenolicum
0.0008
-
Pentachlorophenol pH 7.0, 25°C, recombinant mutant R235K Sphingobium chlorophenolicum
0.0029
-
Pentachlorophenol pH 7.0, 25°C, recombinant mutant Y39F Sphingobium chlorophenolicum
0.0045
-
Pentachlorophenol pH 7.0, 25°C, recombinant mutant Y216F Sphingobium chlorophenolicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pentachlorophenol + NADPH + H+ + O2 Sphingobium chlorophenolicum
-
tetrachlorobenzoquinone + NADP+ + chloride + H2O
-
ir

Organism

Organism UniProt Comment Textmining
Sphingobium chlorophenolicum
-
strain ATCC 39723
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant enzymes from Escherichia coli Sphingobium chlorophenolicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Sphingobium chlorophenolicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pentachlorophenol + NADPH + H+ + O2
-
Sphingobium chlorophenolicum tetrachlorobenzoquinone + NADP+ + chloride + H2O
-
ir

Subunits

Subunits Comment Organism
? x * 62600-62750, about, native wild-type enzyme, and recombinant wild-type and mutant enzymes, overview Sphingobium chlorophenolicum
More 3D-structure of the enzyme Sphingobium chlorophenolicum

Synonyms

Synonyms Comment Organism
PcpB
-
Sphingobium chlorophenolicum
pentachlorophenol 4-monooxygenase
-
Sphingobium chlorophenolicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Sphingobium chlorophenolicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Sphingobium chlorophenolicum
4100
-
Pentachlorophenol pH 7.0, 25°C, recombinant mutant Y216F Sphingobium chlorophenolicum
9300
-
Pentachlorophenol pH 7.0, 25°C, recombinant wild-type enzyme Sphingobium chlorophenolicum
15200
-
Pentachlorophenol pH 7.0, 25°C, recombinant mutant R235K Sphingobium chlorophenolicum
16000
-
Pentachlorophenol pH 7.0, 25°C, recombinant mutant Y39F Sphingobium chlorophenolicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Sphingobium chlorophenolicum

Cofactor

Cofactor Comment Organism Structure
FAD flavoenzyme, O2 binds to FAD and reduces it Sphingobium chlorophenolicum
NADPH
-
Sphingobium chlorophenolicum