Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression of His-tagged wild-type and mutant enzymes in Escherichia coli | Sphingobium chlorophenolicum |
Protein Variants | Comment | Organism |
---|---|---|
F85A | site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme | Sphingobium chlorophenolicum |
R235A | site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme | Sphingobium chlorophenolicum |
R235E | site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme | Sphingobium chlorophenolicum |
R235K | site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme | Sphingobium chlorophenolicum |
Y216A | site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme | Sphingobium chlorophenolicum |
Y397A | site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme | Sphingobium chlorophenolicum |
Y397F | site-directed mutagenesis, determination of molecular weight and comparison to wild-type enzyme | Sphingobium chlorophenolicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Sphingobium chlorophenolicum | |
0.0005 | - |
Pentachlorophenol | pH 7.0, 25°C, recombinant wild-type enzyme | Sphingobium chlorophenolicum | |
0.0008 | - |
Pentachlorophenol | pH 7.0, 25°C, recombinant mutant R235K | Sphingobium chlorophenolicum | |
0.0029 | - |
Pentachlorophenol | pH 7.0, 25°C, recombinant mutant Y39F | Sphingobium chlorophenolicum | |
0.0045 | - |
Pentachlorophenol | pH 7.0, 25°C, recombinant mutant Y216F | Sphingobium chlorophenolicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pentachlorophenol + NADPH + H+ + O2 | Sphingobium chlorophenolicum | - |
tetrachlorobenzoquinone + NADP+ + chloride + H2O | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingobium chlorophenolicum | - |
strain ATCC 39723 | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli | Sphingobium chlorophenolicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Sphingobium chlorophenolicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pentachlorophenol + NADPH + H+ + O2 | - |
Sphingobium chlorophenolicum | tetrachlorobenzoquinone + NADP+ + chloride + H2O | - |
ir |
Subunits | Comment | Organism |
---|---|---|
? | x * 62600-62750, about, native wild-type enzyme, and recombinant wild-type and mutant enzymes, overview | Sphingobium chlorophenolicum |
More | 3D-structure of the enzyme | Sphingobium chlorophenolicum |
Synonyms | Comment | Organism |
---|---|---|
PcpB | - |
Sphingobium chlorophenolicum |
pentachlorophenol 4-monooxygenase | - |
Sphingobium chlorophenolicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Sphingobium chlorophenolicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Sphingobium chlorophenolicum | |
4100 | - |
Pentachlorophenol | pH 7.0, 25°C, recombinant mutant Y216F | Sphingobium chlorophenolicum | |
9300 | - |
Pentachlorophenol | pH 7.0, 25°C, recombinant wild-type enzyme | Sphingobium chlorophenolicum | |
15200 | - |
Pentachlorophenol | pH 7.0, 25°C, recombinant mutant R235K | Sphingobium chlorophenolicum | |
16000 | - |
Pentachlorophenol | pH 7.0, 25°C, recombinant mutant Y39F | Sphingobium chlorophenolicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Sphingobium chlorophenolicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoenzyme, O2 binds to FAD and reduces it | Sphingobium chlorophenolicum | |
NADPH | - |
Sphingobium chlorophenolicum |