Crystallization (Comment) | Organism |
---|---|
- |
Flavobacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
Pentachlorophenol | - |
Flavobacterium sp. | |
0.082 | - |
NADPH | - |
Flavobacterium sp. | |
0.214 | - |
NADH | - |
Flavobacterium sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for divalent cations | Flavobacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63000 | - |
1 * 63000, SDS-PAGE, under certain conditions dimer and multimer conformations are also observed | Flavobacterium sp. |
66000 | - |
gel filtration | Flavobacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flavobacterium sp. | - |
strain ATCC 39723 | - |
Purification (Comment) | Organism |
---|---|
- |
Flavobacterium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Flavobacterium sp. |
Storage Stability | Organism |
---|---|
-20°C, as ammonium sulfate precipitate or in solution at concentration greater than 1 mg/ml, 2 months | Flavobacterium sp. |
4°C, 50% loss of activity after 1 week | Flavobacterium sp. |
4°C, in cell extract or as 40-60% fraction of ammonium sulfate precipitation, total loss of activity after 3 days | Flavobacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pentachlorophenol + NADH + H+ + O2 | - |
Flavobacterium sp. | 2,3,5,6-tetrachloro-1,4-benzoquinone + NAD+ + chloride + H2O | - |
r | |
pentachlorophenol + NADPH + H+ + O2 | - |
Flavobacterium sp. | 2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O | - |
? | |
triiodophenol + NADPH + O2 | - |
Flavobacterium sp. | diiodohydroquinone + iodide + NADP+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 63000, SDS-PAGE, under certain conditions dimer and multimer conformations are also observed | Flavobacterium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Flavobacterium sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 45 | 40°C: activity maximum, 45°C: above 75% of activity maximum, 50°C: inactive | Flavobacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Flavobacterium sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | pH 6.0: about 28% of activity maximum, pH 7.5-8.5: activity maximum | Flavobacterium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1 FAD per enzyme molecule | Flavobacterium sp. | |
additional information | dithiothreitol can not be used as electron donor | Flavobacterium sp. | |
NADH | can replace NADPH, less effective | Flavobacterium sp. | |
NADPH | 2 mol of NADPH per mol of halogenated substrate, preferred electron donor | Flavobacterium sp. |