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Literature summary for 1.14.13.50 extracted from

  • Xun, L.; Orser, C.S.
    Purification and properties of pentachlorophenol hydroxylase, a flavoprotein from Flavobacterium sp. strain ATCC 39723 [published erratum appears in J Bacteriol 1992 Aug;174(15):5176] (1991), J. Bacteriol., 173, 4447-4453.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Flavobacterium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.03
-
Pentachlorophenol
-
Flavobacterium sp.
0.082
-
NADPH
-
Flavobacterium sp.
0.214
-
NADH
-
Flavobacterium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no requirement for divalent cations Flavobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
1 * 63000, SDS-PAGE, under certain conditions dimer and multimer conformations are also observed Flavobacterium sp.
66000
-
gel filtration Flavobacterium sp.

Organism

Organism UniProt Comment Textmining
Flavobacterium sp.
-
strain ATCC 39723
-

Purification (Commentary)

Purification (Comment) Organism
-
Flavobacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Flavobacterium sp.

Storage Stability

Storage Stability Organism
-20°C, as ammonium sulfate precipitate or in solution at concentration greater than 1 mg/ml, 2 months Flavobacterium sp.
4°C, 50% loss of activity after 1 week Flavobacterium sp.
4°C, in cell extract or as 40-60% fraction of ammonium sulfate precipitation, total loss of activity after 3 days Flavobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pentachlorophenol + NADH + H+ + O2
-
Flavobacterium sp. 2,3,5,6-tetrachloro-1,4-benzoquinone + NAD+ + chloride + H2O
-
r
pentachlorophenol + NADPH + H+ + O2
-
Flavobacterium sp. 2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
?
triiodophenol + NADPH + O2
-
Flavobacterium sp. diiodohydroquinone + iodide + NADP+ + H2O
-
?

Subunits

Subunits Comment Organism
monomer 1 * 63000, SDS-PAGE, under certain conditions dimer and multimer conformations are also observed Flavobacterium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Flavobacterium sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 45 40°C: activity maximum, 45°C: above 75% of activity maximum, 50°C: inactive Flavobacterium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
Flavobacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 pH 6.0: about 28% of activity maximum, pH 7.5-8.5: activity maximum Flavobacterium sp.

Cofactor

Cofactor Comment Organism Structure
FAD 1 FAD per enzyme molecule Flavobacterium sp.
additional information dithiothreitol can not be used as electron donor Flavobacterium sp.
NADH can replace NADPH, less effective Flavobacterium sp.
NADPH 2 mol of NADPH per mol of halogenated substrate, preferred electron donor Flavobacterium sp.