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Literature summary for 1.14.13.50 extracted from

  • Schenk, T.; Muller, R.; Mörsberger, F.; Otto, M.K.; Lingens, F.
    Enzymatic dehalogenation of pentachlorophenol by extracts from Arthrobacter sp. strain ATCC 33790 (1989), J. Bacteriol., 171, 5487-5491.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.13.50

Activating Compound

Activating Compound Comment Organism Structure
EDTA 0.01 mM, stimulates by 67% Arthrobacter sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information probably not a soluble protein Arthrobacter sp.
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ stimulates Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
 strain ATCC 33790
-

Purification (Commentary)

Purification (Comment) Organism
partially Arthrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activity in crude extracts is undetectable or below the detection limit of 0.1 nU per mg with a chloride sensor Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3,4,5-tetrachlorophenol + NADPH + O2
-
 Arthrobacter sp. 2,3,5-trichlorohydroquinone + Cl- + NADP+ + H2O
-
 ?
2,3,4-trichlorophenol + NADPH + O2
-
 Arthrobacter sp. ?
-
 ?
2,4,5-trichlorophenol + NADPH + O2
-
 Arthrobacter sp. ?
-
 ?
2,4,6-trichlorophenol + NADPH + O2
-
 Arthrobacter sp. 2,6-dichloro-p-hydroquinone + Cl- + NADP+ + H2O
-
 ?
additional information no activity with 3,4,5-trichlorophenol, 2,4-dichlorophenol, 3,4-dichlorophenol, 4-chlorophenol Arthrobacter sp. ?
-
 ?
pentachlorophenol + NADPH + H+ + O2 oxygen and NADPH are essential Arthrobacter sp. 2,3,5,6-tetrachlorohydroquinone + NADP+ + chloride + H2O
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
 Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Arthrobacter sp.

Cofactor

Cofactor Comment Organism Structure
NADPH essential Arthrobacter sp.