Crystallization (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
mercury compounds | - |
Pseudomonas sp. | |
p-chloromercuribenzoate | - |
Pseudomonas sp. | |
silver compounds | - |
Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
NADH | - |
Pseudomonas sp. | |
0.002 | 0.01 | NADH | - |
Pseudomonas sp. | |
0.02 | - |
O2 | - |
Pseudomonas sp. | |
0.3 | - |
imidazoleacetate | - |
Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
87000 | 90000 | sedimentation equilibrium, gel filtration | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-imidazoleacetate + NADH + O2 | Pseudomonas sp. | - |
5-hydroxy-4-imidazoleacetate + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using protamine sulfate treatment, ammonium sulfate fractionation, chromatography on TEAE-cellulose column, calcium phosphate gel treatment and chromatography on DEAE-Sephadex column | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-20°C, potassium phosphate buffer, pH 7.2, no loss of activity for at least 1 month | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-imidazoleacetate + NAD(P)H + O2 + H+ | - |
Pseudomonas sp. | 5-hydroxy-4-imidazoleacetate + NAD(P)+ + H2O | - |
? | |
4-imidazoleacetate + NADH + O2 | - |
Pseudomonas sp. | 5-hydroxy-4-imidazoleacetate + NAD+ + H2O | - |
? | |
imidazolelactate + NADH + O2 + H+ | 4% relative activity to 4-imidazoleacetate | Pseudomonas sp. | 5-hydroxyimidazolelactate + NAD+ + H2O | - |
? | |
imidazolepropionate + NAD(P)H + O2 + H+ | 10% relative activity to 4-imidazoleacetate | Pseudomonas sp. | 5-hydroxyimidazolepropionate + NAD(P)+ + H2O | - |
? | |
sodium diethyldithiocarbamate + NADH + O2 | - |
Pseudomonas sp. | ? + NAD+ + H2O | reaction product not identified | ? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
50% loss of activity, 0.1 M potassium phosphate, pH 7.2, 5 min | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1 mol per mol enzyme | Pseudomonas sp. | |
NADH | - |
Pseudomonas sp. | |
NADPH | beta-NADPH is approximately 13% as active as beta-NADH | Pseudomonas sp. | |
NADPH | less efficient than NADH | Pseudomonas sp. |