Cloned (Comment) | Organism |
---|---|
expression of nNOS in Escherichia coli | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics, rapid-freeze method, overview | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | dependent on | Rattus norvegicus | |
Fe2+ | a heme enzyme | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | Rattus norvegicus | - |
2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P29476 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant nNOS from Escherichia coli in absence of tetrahydrobiopterin | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
neuron | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 2 NADPH + 2 H+ + 2 O2 | first half reaction via intermediate Nomega-hydroxy-L-arginine | Rattus norvegicus | 2 Nomega-hydroxy-L-arginine + 2 NADP+ + 2 H2O | - |
? | |
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | - |
Rattus norvegicus | 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 | complete reaction, during Arg hydroxylation, H4B acts as a one-electron donor and is then presumed to redox cycle, i.e. be reduced back to H4B, within NOS before further catalysis can proceed. Calmodulin-dependent reduction of a tetrahydrobiopterin radical, mechanism involving the NOS flavoprotein domain, reaction scheme, overview | Rattus norvegicus | 2 citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | - |
? | |
additional information | nitric-oxide synthases are catalytically self-sufficient flavo-heme enzymes that generate NO from L-arginine and display an utilization of their tetrahydrobiopterin cofactor, overview | Rattus norvegicus | ? | - |
? | |
Nomega-hydroxy-L-arginine + NADPH + H+ + O2 | second half reaction via intermediate Nomega-hydroxy-L-arginine | Rattus norvegicus | citrulline + nitric oxide + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
neuronal NOS | - |
Rattus norvegicus |
NOS | - |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | dependent on | Rattus norvegicus | |
FMNH2 | - |
Rattus norvegicus | |
heme | - |
Rattus norvegicus | |
NADPH | - |
Rattus norvegicus | |
tetrahydrobiopterin | - |
Rattus norvegicus |