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Literature summary for 1.14.13.39 extracted from

  • Wang, W.S.; Hung, S.W.; Lin, Y.H.; Tu, C.Y.; Wong, M.L.; Chiou, S.H.; Shieh, M.T.
    Purification and localization of nitric oxide synthases from hybrid tilapia (Nile tilapia x Mozambique tilapia) (2007), J. Aquat. Anim. Health, 19, 168-178.
    View publication on PubMed
Search for more literature for 1.14.13.39

Inhibitors

Inhibitors Comment Organism Structure
Gly-methyl-L-arginine inhibition of the isozymes in absence or presence of L-arginine Oreochromis mossambicus x niloticus
L-Asn-methyl-L-arginine inhibition of the isozymes in absence or presence of L-arginine Oreochromis mossambicus x niloticus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates both isozymes Oreochromis mossambicus x niloticus
Mg2+ activates both isozymes Oreochromis mossambicus x niloticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
89000
-
 2 * 89000, nNOS, SDS-PAGE, bands of 47 kDa and 29 kDa also occur Oreochromis mossambicus x niloticus
120000
-
 isozyme iNOS, gel filtration Oreochromis mossambicus x niloticus
178000
-
 isozyme nNOS, gel filtration Oreochromis mossambicus x niloticus

Organism

Organism UniProt Comment Textmining
Oreochromis mossambicus x niloticus
-
 hybrid tilapia, three isozymes eNOS, iNOS and nNOS
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from tilapia, by 2',5'-ADP affinity chromatography, ion exchange chromatography, and gel filtration Oreochromis mossambicus x niloticus

Source Tissue

Source Tissue Comment Organism Textmining
cerebellum nNOS Oreochromis mossambicus x niloticus
-
cerebrum nNOS Oreochromis mossambicus x niloticus
-
diencephalon nNOS Oreochromis mossambicus x niloticus
-
endothelium eNOS, endothelial cells of blood vessels Oreochromis mossambicus x niloticus
-
heart eNOS Oreochromis mossambicus x niloticus
-
kidney anterior, iNOS Oreochromis mossambicus x niloticus
-
macrophage iNOS Oreochromis mossambicus x niloticus
-
additional information no activity in gill, intestine, and liver Oreochromis mossambicus x niloticus
-
neuron nNOS Oreochromis mossambicus x niloticus
-
olfactory bulb nNOS Oreochromis mossambicus x niloticus
-
spinal cord nNOS, nerve cell bodies and neuronal fibers Oreochromis mossambicus x niloticus
-
spleen iNOS Oreochromis mossambicus x niloticus
-

Subunits

Subunits Comment Organism
dimer 2 * 89000, nNOS, SDS-PAGE, bands of 47 kDa and 29 kDa also occur Oreochromis mossambicus x niloticus
monomer 1 * 120000, iNOS, SDS-PAGE Oreochromis mossambicus x niloticus

Synonyms

Synonyms Comment Organism
endothelial NOS
-
 Oreochromis mossambicus x niloticus
eNOS
-
 Oreochromis mossambicus x niloticus
inducible NOS
-
 Oreochromis mossambicus x niloticus
iNOS
-
 Oreochromis mossambicus x niloticus
neuronal NOS
-
 Oreochromis mossambicus x niloticus
nNOS
-
 Oreochromis mossambicus x niloticus
NOS
-
 Oreochromis mossambicus x niloticus