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Literature summary for 1.14.13.39 extracted from

  • Wang, Z.Q.; Wei, C.C.; Sharma, M.; Pant, K.; Crane, B.R.; Stuehr, D.J.
    A conserved Val to Ile switch near the heme pocket of animal and bacterial nitric-oxide synthases helps determine their distinct catalytic profiles (2004), J. Biol. Chem., 279, 19018-19025.
    View publication on PubMed
Search for more literature for 1.14.13.39

Cloned(Commentary)

Cloned (Comment) Organism
-
 Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
I224V little effect on binding of L-arginie, tetrahydrobiopterin or in electronic properties Bacillus subtilis
I224V mutation does not greatly alter binding of Arg, (6R)-tetrahydrobiopterin, or alter the electronic properties of the heme or various heme-ligand complexes. I224V displays three heme transitions involving four species as typically occurs in wild-type NOS, the beginning ferrous enzyme, a ferrous-dioxy (FeIIO2) intermediate, FeIIINO, and an ending ferric enzyme. The rate of each transition is increased relative to wild-type, with FeIIINO dissociation being 3.6 times faster Bacillus subtilis
V346I slower NO-binding and dissociation than wild-type Mus musculus

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis O34453
-
-
Bacillus subtilis 168 O34453
-
-
Mus musculus P29477 inducible oxygenase domain
-

Reaction

Reaction Comment Organism Reaction ID
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O analysis of transition states via stopped-flow spectroscopy Bacillus subtilis
a
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O analysis of transition states via stopped-flow spectroscopy Mus musculus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-hydroxy-L-arginine + NADPH + O2
-
 Bacillus subtilis ? + NO + NADP+
-
 ?
N-hydroxy-L-arginine + NADPH + O2
-
 Mus musculus ? + NO + NADP+
-
 ?

Cofactor

Cofactor Comment Organism Structure
5,6,7,8-tetrahydro-L-biopterin
-
 Bacillus subtilis
5,6,7,8-tetrahydro-L-biopterin
-
 Mus musculus
NADPH
-
 Bacillus subtilis
NADPH
-
 Mus musculus