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Literature summary for 1.14.13.39 extracted from

  • Richards, M.K.; Clague, M.J.; Marletta, M.A.
    Characterization of C415 mutants of neuronal nitric oxide synthase (1996), Biochemistry, 35, 7772-7780.
    View publication on PubMed
Search for more literature for 1.14.13.39

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol
-
 Mus musculus

Cloned(Commentary)

Cloned (Comment) Organism
expression of neuronal isoform wild type and mutants C415H, C415A in Spodoptera frugiperda cells via baculovirus infection Mus musculus

Protein Variants

Protein Variants Comment Organism
C415A contains no heme, no bound tetrahydrobiopterin, unable to oxidize NADPH and to synthezise nitric oxide, unaltered ability to reduce cytochrome c Mus musculus
C415H contains nearly no heme, no bound tetrahydrobiopterin, unable to oxidize NADPH and to synthezise nitric oxide, unaltered ability to reduce cytochrome c Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
 Mus musculus
Iron heme ligand is bound via C415 Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type and mutants from insect cells Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
macrophage
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 wild-type and mutants Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
 Mus musculus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
 ?
oxidized cytochrome c + NADPH + O2 wild-type and mutants Mus musculus reduced cytochrome c + NADP+ + H2O
-
 ?

Cofactor

Cofactor Comment Organism Structure
5,6,7,8-tetrahydro-L-biopterin only wild-type Mus musculus
Calmodulin Ca2+/calmodulin stimulates cytochrome c reductase activity Mus musculus
Calmodulin 15fold stimulation of cytochrome c reduction of wild-type and mutants C415A and C415H Mus musculus
FAD wild-type and mutant C415H contain1 mol per mol of subunit Mus musculus
FMN wild-type and mutant C415H contain 0.8 and 0.9 mol per mol of subunit, respectively Mus musculus
NADPH
-
 Mus musculus