Application | Comment | Organism |
---|---|---|
pharmacology | NO synthase can be used to gain insight into the biological role of endogenous agmatine | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
neuronal enzyme expressed in Spodoptera frugiperda cells via baculovirus infection | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
agmatine | at lower concentration than the Ki value agmatine leads to time-, concentration-, NADPH- and calmodulin-dependent inhibition of the neuronal enzyme in presence of calmodulin; causes an increase in NADPH oxidase activity of the enzyme | Homo sapiens | |
H2O2 | alters heme group, decrease in activity | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | 0.88 mol per mol of recombinant enzyme monomer | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type recombinant from insect cells | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
neuron | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
assay method | Homo sapiens |
1 | - |
purified enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Homo sapiens | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.66 | - |
agmatine | at lower concentration than the Ki value agmatine leads to time-, concentration-, NADPH- and calmodulin-dependent inhibition of the neuronal enzyme in presence of calmodulin | Homo sapiens |