Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.13.39 extracted from

  • Campos, K.L.; Giovanelli, J.; Kaufman, S.
    Characteristics of the nitric oxide synthase-catalyzed conversion of arginine to N-hydroxyarginine, the first oxygenation step in the enzymic synthesis of nitric oxide (1995), J. Biol. Chem., 270, 1721-1728.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
rat brain enzyme expressed in human 293 kidney cells transfected with a vector encoding rat brain enzyme Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
cyanide pretreatment Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron
-
Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
cerebellum wild-type and recombinant brain enzyme Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-
cerebellum
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ the overall reaction proceeds via 2 partial reactions: reaction 1 converts L-arginine into L-Ngamma-hydroxyarginine, reaction 2 converts L-Ngamma-hydroxyarginine into citrulline and nitric oxide Rattus norvegicus 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
ir
Ngamma-hydroxy-L-arginine + NADPH + O2 substrate is intermediate between reaction 1 and 2 to form citrulline and NO from L-arginine Rattus norvegicus citrulline + NADP+ + NO
-
ir

Cofactor

Cofactor Comment Organism Structure
5,6,7,8-tetrahydro-L-biopterin required Rattus norvegicus
NADPH
-
Rattus norvegicus