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Literature summary for 1.14.13.39 extracted from

  • Xia, Y.; Tsai, A.L.; Berka, V.; Zweier, J.L.
    Superoxide generation from endothelial nitric-oxide synthase. A Ca2+/calmodulin-dependent and tetrahydrobiopterin regulatory process (1998), J. Biol. Chem., 273, 25804-25808.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
wild-type endothelial enzyme is expressed in insect cells via baculovirus infection Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
cyanide heme-blocker inhibits superoxide formation after pretreatment of the enzyme Homo sapiens
imidazole heme-blocker inhibits superoxide formation after pretreatment of the enzyme Homo sapiens
Ngamma-nitro-L-arginine methyl ester only L-isomer, inhibits NO and citrulline production from L-arginine as well as superoxide formation in absence of tetrahydropterin Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Ca2+/calmodulin is required for citrulline/NO formation and for superoxide formation in absence of tetrahydropterin Homo sapiens
Iron
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
135000
-
x * 135000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
wild-type recombinant from insect cells Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
endothelium
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 L-arginine + 3 NADPH + 4 O2 + 3 H+
-
Homo sapiens 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O
-
?
additional information enzyme can also Ca2+/calmodulin-dependently produce superoxide in absence of tetrahydropterin and in depletion of L-arginine, which is inhibited by tetrahydropterin, cyanide and imidazole Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
? x * 135000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
23
-
about, assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
Calmodulin Ca2+/calmodulin is required for superoxide formation in absence of tetrahydropterin Homo sapiens
NADPH
-
Homo sapiens