Literature summary for 1.14.13.39 extracted from

Kosuga, K.; Yui, Y.; Hattori, R.; Eizawa, H.;Hiki, K.; Kawai, C.
Stabilizing factor(s) of nitric oxide (NO) synthetase (1990), Biochem. Biophys. Res. Commun., 172, 705-708.

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General Stability

General Stability	Organism
heat-, diethylether- and alkali-labile, slightly acid resistant cytosolic factor of MW above 100000 and of little charge at neutral pH stabilizes	Rattus norvegicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Rattus norvegicus	5829	-

Organism

Organism	UniProt	Comment	Textmining
Rattus norvegicus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
2',5'-ADP-affinity and anion exchange chromatography	Rattus norvegicus

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Rattus norvegicus	-
neutrophil	polymorphonuclear	Rattus norvegicus	-
neutrophil	peritoneal polymorphonuclear	Rattus norvegicus	-
peritoneum	-	Rattus norvegicus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.9	-	purified enzyme	Rattus norvegicus

Storage Stability

Storage Stability	Organism
4°C, in the presence of one or more cytosolic factors at least 24 h stable	Rattus norvegicus
4°C, purified, t1/2: 3 h at pH 7.4	Rattus norvegicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 L-arginine + 3 NADPH + 4 O2 + 3 H+	-	Rattus norvegicus	2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O	-	?

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Rattus norvegicus

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
7.4	-	purified, t1/2: 3 h, 4°C	Rattus norvegicus

Cofactor

Cofactor	Comment	Organism	Structure
5,6,7,8-tetrahydro-L-biopterin	-	Rattus norvegicus
Calmodulin	rat neutrophil enzyme is calmodulin independent	Rattus norvegicus
NADPH	-	Rattus norvegicus

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Release 2023.1 (January 2023)