Inhibitors | Comment | Organism | Structure |
---|---|---|---|
nitroblue tetrazolium | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | 0.0084 | dichlorophenolindophenol | - |
Sus scrofa | |
0.016 | - |
nitroblue tetrazolium | - |
Sus scrofa | |
0.0323 | 0.035 | L-arginine | cytochrome c reduction | Sus scrofa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | the enzyme bears Ca2+/calmodulin dependent cytochrome P-450 reductase activity which catalyzes cytochrome c reduction | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Sus scrofa | NO represents the endogenous activator of soluble guanylyl cyclase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Sus scrofa | - |
cerebellum | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.01 | - |
reductase activity | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Sus scrofa | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | the product is a guanylyl-cyclase-relaxing factor, that is identical with nitric oxide or a NO-releasing compound | ? | |
2,6-dichlorophenolindophenol + NADPH + O2 | - |
Sus scrofa | ? + NO + NADP+ | - |
? | |
additional information | the reductase domain has a broad substrate specificity, catalyzes a moderate Ca2+/calmodulin independent hydroxylation when the enzyme is reconstituted with purified P-450 | Sus scrofa | ? | - |
? | |
additional information | NO represents the endogenous activator of soluble guanylyl cyclase | Sus scrofa | ? | - |
? | |
nitroblue tetrazolium + 2 NADPH | - |
Sus scrofa | nitroblue tetrazolium formazan + 2 NADP+ | - |
? | |
oxidized cytochrome c + NADPH + O2 | reaction is enhanced by addition of calmodulin at 0.0002 mM | Sus scrofa | reduced cytochrome c + NADP+ + H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
2,6-dichlorophenolindophenol | activation | Sus scrofa | |
5,6,7,8-tetrahydro-L-biopterin | - |
Sus scrofa | |
Calmodulin | the enzyme bears a Ca2+/calmodulin dependent FAD and FMN containing reductase domain which transfers electrons from NADPH to a variety of acceptors | Sus scrofa | |
cytochrome c | activation | Sus scrofa | |
FAD | the enzyme bears Ca2+/calmodulin dependent FAD and FMN containing reductase domain which transfers electrons from NADPH to a variety of acceptors | Sus scrofa | |
FMN | the enzyme bears Ca2+/calmodulin dependent FAD and FMN containing reductase domain which transfers electrons from NADPH to a variety of acceptors | Sus scrofa | |
NADPH | - |
Sus scrofa | |
nitroblue tetrazolium | activation | Sus scrofa |