Cloned (Comment) | Organism |
---|---|
rat brain enzyme expressed in human 293 kidney cells transfected with a vector encoding rat brain enzyme | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CO | - |
Mus musculus | |
CO | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | 2 mol iron-protoporphyrin IX per mol enzyme dimer, the heme-iron is ferric, EPR-and light absorbance spectroscopy | Mus musculus | |
Iron | 2 mol iron-protoporphyrin IX per mol enzyme dimer, the heme-iron is ferric, EPR-and light absorbance spectroscopy | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | Mus musculus | acts as signal molecule for neurotransmission, vasorelaxation, and cytotoxity | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | Rattus norvegicus | acts as signal molecule for neurotransmission, vasorelaxation, and cytotoxity | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
2',5'-ADP-agarose affinity chromatography | Rattus norvegicus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | cytochrome P-450 enzyme | Mus musculus | |
2 L-arginine + 3 NADPH + 3 H+ + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O | cytochrome P-450 enzyme | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
brain | - |
Rattus norvegicus | - |
macrophage | cytokine-activated | Mus musculus | - |
macrophage | cytokine-activated | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Mus musculus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | probably via Nomega-hydroxy-L-arginine | ? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | - |
Rattus norvegicus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | probably via Nomega-hydroxy-L-arginine | ? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | acts as signal molecule for neurotransmission, vasorelaxation, and cytotoxity | Mus musculus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? | |
2 L-arginine + 3 NADPH + 4 O2 + 3 H+ | acts as signal molecule for neurotransmission, vasorelaxation, and cytotoxity | Rattus norvegicus | 2 L-citrulline + 2 NO + 3 NADP+ + 4 H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
37 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | FAD containing flavoprotein | Mus musculus | |
FAD | FAD containing flavoprotein | Rattus norvegicus | |
FMN | FMN containing flavoprotein | Mus musculus | |
FMN | FMN containing flavoprotein | Rattus norvegicus | |
NADPH | - |
Mus musculus | |
NADPH | - |
Rattus norvegicus |