Cloned (Comment) | Organism |
---|---|
gene otcC, DNA and amino acid sequence determination and analysis, expression of the glycine mutant enzyme in Escherichia coli strain BL21(DE3) | Streptomyces rimosus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | a otcC disruption mutant strain of Streptomyces rimosus synthesizes a novel C-17 polyketide, the ability to make a 19-carbon backbone in the mutant strain is restored when the inactive mutant enzyme, with three essential glycine residues of the NADH-binding domain mutated by site-directed mutagenesis, is expressed in the disruption mutant strain, thus the quarternary structure of the enzyme is required, not only the its activity, in the synthase complex | Streptomyces rimosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
anhydrotetracycline + NADPH + O2 | Streptomyces rimosus | the enzyme catalyzes a hydroxylation of the anthracycline structure at position C-6 after biosynthesis of the polyketide backbone is completed, Biosynthesis of oxytetracycline, overview | 12-dehydrotetracycline + NADP+ + H2O | product identification | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces rimosus | Q58PK7 | gene otcC, strain R6-500 | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000112 | - |
recombinant wild-type enzyme in Escherichia coli cell extract | Streptomyces rimosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
anhydrotetracycline + NADPH + O2 | - |
Streptomyces rimosus | 12-dehydrotetracycline + NADP+ + H2O | - |
? | |
anhydrotetracycline + NADPH + O2 | the enzyme catalyzes a hydroxylation of the anthracycline structure at position C-6 after biosynthesis of the polyketide backbone is completed, Biosynthesis of oxytetracycline, overview | Streptomyces rimosus | 12-dehydrotetracycline + NADP+ + H2O | product identification | ? |
Synonyms | Comment | Organism |
---|---|---|
anhydrotetracycline oxygenase | - |
Streptomyces rimosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | three essential glycine codons are located within the putative NADPH-binding domain | Streptomyces rimosus |