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Literature summary for 1.14.13.35 extracted from

  • Sreeleela, N.S.; SubraRao, P.V.; Premkumar, R.; Vaidyanathan, C.S.
    A new anthranilic acid hydroxylase from Aspergillus niger. Purification and properties (1969), J. Biol. Chem., 244, 2293-2298.
    View publication on PubMed

General Stability

General Stability Organism
glutathione stabilizes Aspergillus niger
the enzyme is inactivated by treatment with ammonium sulfate or by adsorption on DEAE-cellulose or CM-cellulose, filtration through Sephadex G-25 or dialysis against 0.025 M sodium phosphate buffer, pH 7, containing 1 mM GSH irreversibly inactivates the enzyme Aspergillus niger

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline 46% inhibition at 0.5 mM, 60% inhibition at 1 mM Aspergillus niger
2,2'-dipyridyl 40% inhibition at 1 mM Aspergillus niger
3-hydroxyanthranilic acid
-
Aspergillus niger
3-Hydroxybenzoic acid
-
Aspergillus niger
8-hydroxyquinoline 37% inhibition at 1 mM Aspergillus niger
Cd2+
-
Aspergillus niger
Cu2+
-
Aspergillus niger
diethyldithiocarbamate
-
Aspergillus niger
heavy metal ions
-
Aspergillus niger
Hg2+
-
Aspergillus niger
additional information not: atebrin, aminopterin Aspergillus niger
N-ethylmaleimide 95% inhibition at 0.5 mM Aspergillus niger
NaN3 42% inhibition at 0.5 mM Aspergillus niger
p-hydroxymercuribenzoate 96% inhibition at 0.5 mM Aspergillus niger
Salicylaldoxime 42% inhibition at 0.5 mM, 58% inhibition at 1 mM Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
anthranilic acid
-
Aspergillus niger
0.16
-
NADPH
-
Aspergillus niger

Metals/Ions

Metals/Ions Comment Organism Structure
Iron Fe2+ probably required for activity Aspergillus niger

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone inactivates the enzyme Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, using protamine sulfate treatment, DEAE-cellulose treatment, alumina C-gamma treatment and hydroxylapatite treatment Aspergillus niger

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
Aspergillus niger
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0507
-
-
Aspergillus niger
0.0507
-
partially purified enzyme Aspergillus niger

Storage Stability

Storage Stability Organism
-20°C, 50% inactivation of the purified enzyme after 2 days Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
anthranilate + NADPH + O2
-
Aspergillus niger 2,3-dihydroxybenzoate + NADP+ + NH3
-
?
additional information no activity with: 3-hydroxyanthranilic acid, benzoic acid, salicylic acid, m-hydroxybenzoic acid, p-hydroxybenzoic acid, m-aminobenzoic acid, p-aminobenzoic acid, methylanthranilate or ethylanthranilate Aspergillus niger ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
-
Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9.5 pH 7.5: about 80% of activity maximum, pH 9.5: about 55% of activity maximum Aspergillus niger

Cofactor

Cofactor Comment Organism Structure
additional information 2% of the activity when NADPH is replaced by NADH, no effect: FAD, FMN Aspergillus niger
NADPH absolute requirement for NADPH Aspergillus niger