General Stability | Organism |
---|---|
glutathione stabilizes | Aspergillus niger |
the enzyme is inactivated by treatment with ammonium sulfate or by adsorption on DEAE-cellulose or CM-cellulose, filtration through Sephadex G-25 or dialysis against 0.025 M sodium phosphate buffer, pH 7, containing 1 mM GSH irreversibly inactivates the enzyme | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 46% inhibition at 0.5 mM, 60% inhibition at 1 mM | Aspergillus niger | |
2,2'-dipyridyl | 40% inhibition at 1 mM | Aspergillus niger | |
3-hydroxyanthranilic acid | - |
Aspergillus niger | |
3-Hydroxybenzoic acid | - |
Aspergillus niger | |
8-hydroxyquinoline | 37% inhibition at 1 mM | Aspergillus niger | |
Cd2+ | - |
Aspergillus niger | |
Cu2+ | - |
Aspergillus niger | |
diethyldithiocarbamate | - |
Aspergillus niger | |
heavy metal ions | - |
Aspergillus niger | |
Hg2+ | - |
Aspergillus niger | |
additional information | not: atebrin, aminopterin | Aspergillus niger | |
N-ethylmaleimide | 95% inhibition at 0.5 mM | Aspergillus niger | |
NaN3 | 42% inhibition at 0.5 mM | Aspergillus niger | |
p-hydroxymercuribenzoate | 96% inhibition at 0.5 mM | Aspergillus niger | |
Salicylaldoxime | 42% inhibition at 0.5 mM, 58% inhibition at 1 mM | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
anthranilic acid | - |
Aspergillus niger | |
0.16 | - |
NADPH | - |
Aspergillus niger |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | Fe2+ probably required for activity | Aspergillus niger |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | inactivates the enzyme | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial, using protamine sulfate treatment, DEAE-cellulose treatment, alumina C-gamma treatment and hydroxylapatite treatment | Aspergillus niger |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Aspergillus niger | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0507 | - |
- |
Aspergillus niger |
0.0507 | - |
partially purified enzyme | Aspergillus niger |
Storage Stability | Organism |
---|---|
-20°C, 50% inactivation of the purified enzyme after 2 days | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
anthranilate + NADPH + O2 | - |
Aspergillus niger | 2,3-dihydroxybenzoate + NADP+ + NH3 | - |
? | |
additional information | no activity with: 3-hydroxyanthranilic acid, benzoic acid, salicylic acid, m-hydroxybenzoic acid, p-hydroxybenzoic acid, m-aminobenzoic acid, p-aminobenzoic acid, methylanthranilate or ethylanthranilate | Aspergillus niger | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 9.5 | pH 7.5: about 80% of activity maximum, pH 9.5: about 55% of activity maximum | Aspergillus niger |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | 2% of the activity when NADPH is replaced by NADH, no effect: FAD, FMN | Aspergillus niger | |
NADPH | absolute requirement for NADPH | Aspergillus niger |