Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | 50% inhibition at 0.25 mM, 80% inhibition at 0.5 mM and 95% inhibition at 1 mM | Aspergillus niger | |
2,2'-dipyridyl | 50% inhibition at 0.5 mM, 80% inhibition at 1 mM | Aspergillus niger | |
8-hydroxyquinoline | 30% inhibition at 0.5 mM, 40% inhibition at 1 mM | Aspergillus niger | |
additional information | not: atebrin, aminopterin | Aspergillus niger |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | contains 2 gatom of non-heme iron per mol | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
2 * 42000, SDS-PAGE | Aspergillus niger |
89000 | - |
gel filtration | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using protamine sulfate treatment, DEAE-cellulose treatment, ammonium sulfate precipitation, fractionation on Biogel P-100 column, successive negative adsorption on alumina-gel, tricalcium phosphate gel and DEAE-cellulose column, and positive adsorption on a DEAE-Sephadex A-50 column | Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.193 | - |
- |
Aspergillus niger |
Storage Stability | Organism |
---|---|
-20°C, ammonium sulfate precipitate, stable for at least 3 months | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
anthranilate + NADPH + O2 | - |
Aspergillus niger | 2,3-dihydroxybenzoate + NADP+ + NH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 42000, SDS-PAGE | Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
half-life: 14 h | Aspergillus niger |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Aspergillus niger | |
NADPH | - |
Aspergillus niger |