General Stability | Organism |
---|---|
2-nitrophenol stabilizes against heat inactivation | Pseudomonas putida |
glycerol stabilizes during storage at -20°C | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,4-dinitrophenol | competitive inhibition | Pseudomonas putida | |
2-Nitrophenol | substrate inhibition above 0.02 mM | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
2-Nitrophenol | - |
Pseudomonas putida | |
0.14 | - |
NADPH | - |
Pseudomonas putida | |
2 | - |
NADH | - |
Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | stimulates | Pseudomonas putida | |
Mn2+ | stimulates | Pseudomonas putida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
gel filtration | Pseudomonas putida |
65000 | - |
SDS-PAGE | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida B2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
strain B2 | Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2 | - |
- |
Pseudomonas putida |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol, less than 25% loss of activity after 5 days | Pseudomonas putida |
4°C, 20 mM phosphate buffer, 50 mM Tris buffer, pH 7.5, 4 mM DTT, less than 35% loss of activity after 5 days | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenol + NADH + H+ + O2 | - |
Pseudomonas putida | catechol + nitrite + NAD+ + H2O | - |
r | |
2-nitrophenol + NADPH + H+ + O2 | - |
Pseudomonas putida | catechol + nitrite + NADP+ + H2O | - |
? | |
2-nitrophenol + NADPH + H+ + O2 | - |
Pseudomonas putida B2 | catechol + nitrite + NADP+ + H2O | - |
? | |
3-methyl-2-nitrophenol + NADPH + O2 | - |
Pseudomonas putida | 3-methylcatechol + NADP+ + H2O | - |
? | |
3-methyl-2-nitrophenol + NADPH + O2 | - |
Pseudomonas putida B2 | 3-methylcatechol + NADP+ + H2O | - |
? | |
4-methyl-2-nitrophenol + NADPH + O2 | - |
Pseudomonas putida | 4-methylcatechol + NADP+ + H2O | - |
? | |
4-methyl-2-nitrophenol + NADPH + O2 | - |
Pseudomonas putida B2 | 4-methylcatechol + NADP+ + H2O | - |
? | |
5-methyl-2-nitrophenol + NADPH + O2 | - |
Pseudomonas putida | 5-methylcatechol + NADP+ + H2O | - |
? | |
5-methyl-2-nitrophenol + NADPH + O2 | - |
Pseudomonas putida B2 | 5-methylcatechol + NADP+ + H2O | - |
? | |
additional information | broad specificity towards alkylated and halogenated o-nitrophenols, overview | Pseudomonas putida | ? | - |
? | |
additional information | broad specificity towards alkylated and halogenated o-nitrophenols, overview | Pseudomonas putida B2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 65000, SDS-PAGE | Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
stable for several hours in 20 mM phosphate buffer or 50 mM Tris buffer | Pseudomonas putida |
40 | - |
2 min, complete inactivation in absence of o-nitrophenol, 2-nitrophenol above 0.2 mM prevents heat inactivation | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | o-nitrophenol, 4-chloro-2-nitrophenol | Pseudomonas putida |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | pH 6.5: about 60% of activity maximum, pH 9.0: about 55% of activity maximum | Pseudomonas putida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | highest stability | Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | very low affinity | Pseudomonas putida | |
NADPH | - |
Pseudomonas putida |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
2,4-dinitrophenol | - |
Pseudomonas putida |