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Literature summary for 1.14.13.31 extracted from

  • Zeyer, J.; Kocher, H.P.
    Purification and characterization of a bacterial nitrophenol oxygenase which converts ortho-nitrophenol to catechol and nitrite (1988), J. Bacteriol., 170, 1789-1794.
    View publication on PubMedView publication on EuropePMC

General Stability

General Stability Organism
2-nitrophenol stabilizes against heat inactivation Pseudomonas putida
glycerol stabilizes during storage at -20°C Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
2,4-dinitrophenol competitive inhibition Pseudomonas putida
2-Nitrophenol substrate inhibition above 0.02 mM Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
2-Nitrophenol
-
Pseudomonas putida
0.14
-
NADPH
-
Pseudomonas putida
2
-
NADH
-
Pseudomonas putida

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ stimulates Pseudomonas putida
Mn2+ stimulates Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
gel filtration Pseudomonas putida
65000
-
SDS-PAGE Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-
Pseudomonas putida B2
-
-
-

Purification (Commentary)

Purification (Comment) Organism
strain B2 Pseudomonas putida

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2
-
-
Pseudomonas putida

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol, less than 25% loss of activity after 5 days Pseudomonas putida
4°C, 20 mM phosphate buffer, 50 mM Tris buffer, pH 7.5, 4 mM DTT, less than 35% loss of activity after 5 days Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenol + NADH + H+ + O2
-
Pseudomonas putida catechol + nitrite + NAD+ + H2O
-
r
2-nitrophenol + NADPH + H+ + O2
-
Pseudomonas putida catechol + nitrite + NADP+ + H2O
-
?
2-nitrophenol + NADPH + H+ + O2
-
Pseudomonas putida B2 catechol + nitrite + NADP+ + H2O
-
?
3-methyl-2-nitrophenol + NADPH + O2
-
Pseudomonas putida 3-methylcatechol + NADP+ + H2O
-
?
3-methyl-2-nitrophenol + NADPH + O2
-
Pseudomonas putida B2 3-methylcatechol + NADP+ + H2O
-
?
4-methyl-2-nitrophenol + NADPH + O2
-
Pseudomonas putida 4-methylcatechol + NADP+ + H2O
-
?
4-methyl-2-nitrophenol + NADPH + O2
-
Pseudomonas putida B2 4-methylcatechol + NADP+ + H2O
-
?
5-methyl-2-nitrophenol + NADPH + O2
-
Pseudomonas putida 5-methylcatechol + NADP+ + H2O
-
?
5-methyl-2-nitrophenol + NADPH + O2
-
Pseudomonas putida B2 5-methylcatechol + NADP+ + H2O
-
?
additional information broad specificity towards alkylated and halogenated o-nitrophenols, overview Pseudomonas putida ?
-
?
additional information broad specificity towards alkylated and halogenated o-nitrophenols, overview Pseudomonas putida B2 ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 65000, SDS-PAGE Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
stable for several hours in 20 mM phosphate buffer or 50 mM Tris buffer Pseudomonas putida
40
-
2 min, complete inactivation in absence of o-nitrophenol, 2-nitrophenol above 0.2 mM prevents heat inactivation Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8 o-nitrophenol, 4-chloro-2-nitrophenol Pseudomonas putida

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9 pH 6.5: about 60% of activity maximum, pH 9.0: about 55% of activity maximum Pseudomonas putida

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5 8 highest stability Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NADH very low affinity Pseudomonas putida
NADPH
-
Pseudomonas putida

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0005
-
2,4-dinitrophenol
-
Pseudomonas putida