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Literature summary for 1.14.13.27 extracted from

  • Tsuji, H.; Ogawa, T.; Bando, N.; Sasaoka, K.
    Purification and properties of 4-aminobenzoate hydroxylase, a new monooxygenase from Agaricus bisporus (1986), J. Biol. Chem., 261, 13203-13209.
    View publication on PubMed
Search for more literature for 1.14.13.27

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 93% inactivation at 0.1 mM Agaricus bisporus
Br- 37% inactivation at 0.1 M Agaricus bisporus
Cl- 30% inactivation at 0.1 M Agaricus bisporus
CuSO4 91% inactivation at 0.1 mM Agaricus bisporus
F- 20% inactivation at 0.1 M Agaricus bisporus
HgCl2 92% inactivation at 0.1 mM Agaricus bisporus
I- 62% inactivation at 0.1 M Agaricus bisporus
NO3- 45% inactivation at 0.1 M Agaricus bisporus
p-chloromercuribenzoate 94% inactivation at 0.1 mM Agaricus bisporus
SCN- 60% inactivation at 0.1 M Agaricus bisporus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0136
-
 NADH
-
 Agaricus bisporus
0.0204
-
 4-Aminobenzoate
-
 Agaricus bisporus
0.133
-
 NADPH
-
 Agaricus bisporus
0.2
-
 O2
-
 Agaricus bisporus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
 gel filtration Agaricus bisporus
50000
-
 SDS-PAGE Agaricus bisporus

Organism

Organism UniProt Comment Textmining
Agaricus bisporus
-
 mushroom
-

Purification (Commentary)

Purification (Comment) Organism
homogeneity Agaricus bisporus

Storage Stability

Storage Stability Organism
-20°C, 50 mM potassium phosphate buffer, pH 7.0, 20% glycerol, 1 mM 2-mercaptoethanol, 0.01 mM FAD, stable for 1 month Agaricus bisporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-5-chlorobenzoate + NAD(P)H + O2
-
 Agaricus bisporus 4-chloro-2-hydroxyaniline + NAD(P)+ + H2O2 + CO2 H2O2 formed simultaneously with hydroxylation ?
3,4-diaminobenzoate + NAD(P)H + O2 17.3% of the reaction with 4-aminobenzoate Agaricus bisporus 2-amino-4-hydroxyaniline + NAD(P)+ + H2O2 + CO2 H2O2 formed simultaneously with hydroxylation ?
4-amino-2-chlorobenzoate + NAD(P)H + O2
-
 Agaricus bisporus 3-chloro-4-hydroxyaniline + NAD(P)+ + H2O2 + CO2 H2O2 formed simultaneously with hydroxylation ?
4-aminobenzoate + NAD(P)H + O2
-
 Agaricus bisporus 4-hydroxyaniline + NAD(P)+ + H2O + CO2
-
 ?
4-aminobenzoate + NADH + H+ + O2
-
 Agaricus bisporus 4-hydroxyaniline + NAD+ + H2O + CO2
-
 r
4-aminosalicylate + NAD(P)H + O2
-
 Agaricus bisporus 3,4-dihydroxyaniline + NAD(P)+ + H2O2 + CO2 H2O2 formed simultaneously with hydroxylation ?
4-hydroxybenzoate + NAD(P)H + O2 8.4% of the reaction with 4-aminobenzoate Agaricus bisporus quinol + NAD(P)+ + H2O2 + CO2 H2O2 formed simultaneously with hydroxylation ?
anthranilate + NAD(P)H + O2
-
 Agaricus bisporus 2-hydroxyaniline + NAD(P)+ + H2O2 + CO2 H2O2 formed simultaneously with hydroxylation ?
additional information several aromatic compounds stimulate NADH oxidation, but no hydroxylated product is formed, all of the O2 consumed is converted to H2O2 Agaricus bisporus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 49000, SDS-PAGE Agaricus bisporus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Agaricus bisporus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 60 min, little loss of activity in presence of 0.1 mM FAD and 0.02% bovine serum albumin Agaricus bisporus
35
-
 10 min, 50 mM potassium phosphate buffer, pH 7.0, 0.1 mM FAD, 0.02% bovine serum albumin, stable Agaricus bisporus
40
-
 10 min, 50 mM potassium phosphate buffer, pH 7.0, 0.1 mM FAD, 0.02% bovine serum albumin, about 70% loss of activity Agaricus bisporus
45
-
 10 min, 50 mM potassium phosphate buffer, pH 7.0, 0.1 mM FAD, 0.02% bovine serum albumin, 97% loss of activity Agaricus bisporus
50
-
 10 min, 50 mM potassium phosphate buffer, pH 7.0, 0.1 mM FAD, 0.02% bovine serum albumin, 98% loss of activity Agaricus bisporus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7 NADPH Agaricus bisporus
6.5 8 NADH Agaricus bisporus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 8 stable Agaricus bisporus

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Agaricus bisporus
FAD 0.91 mol of FAD per mol of enzyme Agaricus bisporus
NADH
-
 Agaricus bisporus
NADPH 68% of the activity with NADH Agaricus bisporus
NADPH 62% of the activity with NADH Agaricus bisporus