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Literature summary for 1.14.13.250 extracted from

  • He, H.; Henderson, A.; Du, Y.; Ryan, K.
    Two-enzyme pathway links L-arginine to nitric oxide in N-nitroso biosynthesis (2019), J. Am. Chem. Soc., 141, 4026-4033 .
    View publication on PubMed
Search for more literature for 1.14.13.250

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces achromogenes subsp. streptozoticus

Protein Variants

Protein Variants Comment Organism
D214A/E215A H311A modification in putative iron-chelating residues, iron content of protein decreases to 0.4 mol per mol of protein Streptomyces achromogenes subsp. streptozoticus
H311A modification in putative iron-chelating residue, iron content of protein decreases to 0.4 mol per mol of protein Streptomyces achromogenes subsp. streptozoticus
H318A modification in putative iron-chelating residue, iron content of protein decreases to 0.4 mol per mol of protein Streptomyces achromogenes subsp. streptozoticus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron StzF is a nonheme iron-dependent enzyme, containing 0.93 mol iron per mol of enzyme Streptomyces achromogenes subsp. streptozoticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Ndelta,Nomega-dihydroxy-Nomega-methyl-L-arginine + O2 Streptomyces achromogenes subsp. streptozoticus
-
 Ndelta-hydroxy-Nomega-methyl-Nomega-nitroso-L-citrulline + H2O
-
 ?
Ndelta-hydroxy-Nomega-methyl-L-arginine + NADH + H+ + O2 Streptomyces achromogenes subsp. streptozoticus
-
 Ndelta,Nomega-dihydroxy-Nomega-methyl-L-arginine + NAD+ + H2O
-
 ?
Nomega-methyl-L-arginine + 2 NADH + 2 H+ + 3 O2 Streptomyces achromogenes subsp. streptozoticus
-
 Ndelta-hydroxy-Nomega-methyl-Nomega-nitroso-L-citrulline + 2 NAD+ + 3 H2O
-
 ?
Nomega-methyl-L-arginine + NADH + H+ + O2 Streptomyces achromogenes subsp. streptozoticus
-
 Ndelta-hydroxy-Nomega-methyl-L-arginine + NAD+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces achromogenes subsp. streptozoticus A0A411MR89
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ndelta,Nomega-dihydroxy-Nomega-methyl-L-arginine + O2
-
 Streptomyces achromogenes subsp. streptozoticus Ndelta-hydroxy-Nomega-methyl-Nomega-nitroso-L-citrulline + H2O
-
 ?
Ndelta-hydroxy-Nomega-methyl-L-arginine + NADH + H+ + O2
-
 Streptomyces achromogenes subsp. streptozoticus Ndelta,Nomega-dihydroxy-Nomega-methyl-L-arginine + NAD+ + H2O
-
 ?
Nomega-methyl-L-arginine + 2 NADH + 2 H+ + 3 O2
-
 Streptomyces achromogenes subsp. streptozoticus Ndelta-hydroxy-Nomega-methyl-Nomega-nitroso-L-citrulline + 2 NAD+ + 3 H2O
-
 ?
Nomega-methyl-L-arginine + NADH + H+ + O2
-
 Streptomyces achromogenes subsp. streptozoticus Ndelta-hydroxy-Nomega-methyl-L-arginine + NAD+ + H2O
-
 ?

Synonyms

Synonyms Comment Organism
StzF
-
 Streptomyces achromogenes subsp. streptozoticus

pH Range

pH Minimum pH Maximum Comment Organism
3 8
-
 Streptomyces achromogenes subsp. streptozoticus
9
-
 loss of activity above Streptomyces achromogenes subsp. streptozoticus

General Information

General Information Comment Organism
metabolism the N-nitroso group of drug streptozocin stems from the biosynthetic reassembly of L-arginine, with the guanidino nitrogens forming a nitrogen-nitrogen bond. Free L-arginine is N-methylated by StzE to give Nomega-monomethyl-L-arginine. This product is then oxidized by StzF generating a urea compound and NO Streptomyces achromogenes subsp. streptozoticus