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Literature summary for 1.14.13.25 extracted from

  • Hakemian, A.S.; Kondapalli, K.C.; Telser, J.; Hoffman, B.M.; Stemmler, T.L.; Rosenzweig, A.C.
    The metal centers of particulate methane monooxygenase from Methylosinus trichosporium OB3b (2008), Biochemistry, 47, 6793-6801.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapour diffusion method with 100 mM cacodylate, pH 6.5, 20% (v/v) PEG 3000, 250 mM magnesium formate or MnCl2 Methylosinus trichosporium

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Methylosinus trichosporium 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ the membrane-bound pMMO contains 4.8 Cu2+ ions per 100 kDa protomer the purified pMMO contains 1.4 Cu2+ ions per 100 kDa protomer, the enzyme contains a dinuclear copper center Methylosinus trichosporium
Fe2+ the purified pMMO contains 7.6 Fe2+ ions per 100 kDa protomer, the membrane-bound pMMO contains 2.1 Fe2+ ions per 100 kDa protomer Methylosinus trichosporium
Zn2+ the purified pMMO contains 1.3 Zn2+ ions per 100 kDa protomer, the membrane-bound pMMO contains 2.7 Zn2+ ions per 100 kDa protomer Methylosinus trichosporium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
1 * 42000 + 1 * 24000 + 1 * 22000, SDS-PAGE Methylosinus trichosporium
24000
-
1 * 42000 + 1 * 24000 + 1 * 22000, SDS-PAGE Methylosinus trichosporium
42000
-
1 * 42000 + 1 * 24000 + 1 * 22000, SDS-PAGE Methylosinus trichosporium

Organism

Organism UniProt Comment Textmining
Methylosinus trichosporium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Source 15Q column chromatography Methylosinus trichosporium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00011
-
purified enzyme, using propylene and duroquinol as substrates Methylosinus trichosporium
0.0029
-
membrane-bound enzyme, using propylene and NADH as substrates Methylosinus trichosporium
0.003
-
membrane-bound enzyme, using propylene and duroquinol as substrates Methylosinus trichosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methane + NAD(P)H + H+ + O2
-
Methylosinus trichosporium methanol + NAD(P)+ + H2O
-
?
propylene + duroquinol + O2
-
Methylosinus trichosporium propylene oxide + reduced duroquinol + H2O
-
?
propylene + NADH + H+ + O2
-
Methylosinus trichosporium propylene epoxide + NAD+ + H2O
-
?

Subunits

Subunits Comment Organism
heterotrimer 1 * 42000 + 1 * 24000 + 1 * 22000, SDS-PAGE Methylosinus trichosporium

Synonyms

Synonyms Comment Organism
particulate methane monooxygenase
-
Methylosinus trichosporium
pMMO
-
Methylosinus trichosporium

Cofactor

Cofactor Comment Organism Structure
NADH
-
Methylosinus trichosporium
NADPH
-
Methylosinus trichosporium