Crystallization (Comment) | Organism |
---|---|
sitting drop vapour diffusion method with 100 mM cacodylate, pH 6.5, 20% (v/v) PEG 3000, 250 mM magnesium formate or MnCl2 | Methylosinus trichosporium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Methylosinus trichosporium | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | the membrane-bound pMMO contains 4.8 Cu2+ ions per 100 kDa protomer the purified pMMO contains 1.4 Cu2+ ions per 100 kDa protomer, the enzyme contains a dinuclear copper center | Methylosinus trichosporium | |
Fe2+ | the purified pMMO contains 7.6 Fe2+ ions per 100 kDa protomer, the membrane-bound pMMO contains 2.1 Fe2+ ions per 100 kDa protomer | Methylosinus trichosporium | |
Zn2+ | the purified pMMO contains 1.3 Zn2+ ions per 100 kDa protomer, the membrane-bound pMMO contains 2.7 Zn2+ ions per 100 kDa protomer | Methylosinus trichosporium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
1 * 42000 + 1 * 24000 + 1 * 22000, SDS-PAGE | Methylosinus trichosporium |
24000 | - |
1 * 42000 + 1 * 24000 + 1 * 22000, SDS-PAGE | Methylosinus trichosporium |
42000 | - |
1 * 42000 + 1 * 24000 + 1 * 22000, SDS-PAGE | Methylosinus trichosporium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylosinus trichosporium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Source 15Q column chromatography | Methylosinus trichosporium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00011 | - |
purified enzyme, using propylene and duroquinol as substrates | Methylosinus trichosporium |
0.0029 | - |
membrane-bound enzyme, using propylene and NADH as substrates | Methylosinus trichosporium |
0.003 | - |
membrane-bound enzyme, using propylene and duroquinol as substrates | Methylosinus trichosporium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methane + NAD(P)H + H+ + O2 | - |
Methylosinus trichosporium | methanol + NAD(P)+ + H2O | - |
? | |
propylene + duroquinol + O2 | - |
Methylosinus trichosporium | propylene oxide + reduced duroquinol + H2O | - |
? | |
propylene + NADH + H+ + O2 | - |
Methylosinus trichosporium | propylene epoxide + NAD+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotrimer | 1 * 42000 + 1 * 24000 + 1 * 22000, SDS-PAGE | Methylosinus trichosporium |
Synonyms | Comment | Organism |
---|---|---|
particulate methane monooxygenase | - |
Methylosinus trichosporium |
pMMO | - |
Methylosinus trichosporium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Methylosinus trichosporium | |
NADPH | - |
Methylosinus trichosporium |