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Literature summary for 1.14.13.25 extracted from

  • Borodina, E.; Nichol, T.; Dumont, M.G.; Smith, T.J.; Murrell, J.C.
    Mutagenesis of the "leucine gate" to explore the basis of catalytic versatility in soluble methane monooxygenase (2007), Appl. Environ. Microbiol., 73, 6460-6467.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
mutants are expressed in sMMO-negative cells of Methylosinus trichosporium Methylosinus trichosporium

Protein Variants

Protein Variants Comment Organism
L110C mutant shows inverted or shifted regioselectivity with naphthalene, biphenyl, and ethylbenzene as a substrate compared to the wild type enzyme Methylosinus trichosporium
L110G mutant shows inverted or shifted regioselectivity with naphthalene, biphenyl, and ethylbenzene as a substrate compared to the wild type enzyme Methylosinus trichosporium
L110R mutant shows inverted or shifted regioselectivity with naphthalene, biphenyl, and ethylbenzene as a substrate compared to the wild type enzyme Methylosinus trichosporium
L110Y mutant shows inverted or shifted regioselectivity with naphthalene, biphenyl and ethylbenzene as a substrate compared to the wild type enzyme Methylosinus trichosporium

Organism

Organism UniProt Comment Textmining
Methylosinus trichosporium
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
biphenyl + NAD(P)H + H+ + O2
-
Methylosinus trichosporium 2-hydroxybiphenyl + 4-hydroxybiphenyl + NAD(P)+ + H2O
-
?
ethylbenzene + NAD(P)H + H+ + O2
-
Methylosinus trichosporium 1-phenylethanol + 3-ethylphenol + 4-ethylphenol + NAD(P)+ + H2O
-
?
methane + NAD(P)H + H+ + O2
-
Methylosinus trichosporium methanol + NAD(P)+ + H2O
-
?
additional information inactive toward anthracene and phenanthrene Methylosinus trichosporium ?
-
?
naphthalene + NAD(P)H + H+ + O2
-
Methylosinus trichosporium alpha-naphthol + beta-naphthol + NAD(P)+ + H2O
-
?
toluene + NAD(P)H + H+ + O2
-
Methylosinus trichosporium benzyl alcohol + cresol + NAD(P)+ + H2O
-
?

Synonyms

Synonyms Comment Organism
sMMO
-
Methylosinus trichosporium
soluble methane monooxygenase
-
Methylosinus trichosporium

Cofactor

Cofactor Comment Organism Structure
NADH
-
Methylosinus trichosporium
NADPH
-
Methylosinus trichosporium