Cloned (Comment) | Organism |
---|---|
mutants are expressed in sMMO-negative cells of Methylosinus trichosporium | Methylosinus trichosporium |
Protein Variants | Comment | Organism |
---|---|---|
L110C | mutant shows inverted or shifted regioselectivity with naphthalene, biphenyl, and ethylbenzene as a substrate compared to the wild type enzyme | Methylosinus trichosporium |
L110G | mutant shows inverted or shifted regioselectivity with naphthalene, biphenyl, and ethylbenzene as a substrate compared to the wild type enzyme | Methylosinus trichosporium |
L110R | mutant shows inverted or shifted regioselectivity with naphthalene, biphenyl, and ethylbenzene as a substrate compared to the wild type enzyme | Methylosinus trichosporium |
L110Y | mutant shows inverted or shifted regioselectivity with naphthalene, biphenyl and ethylbenzene as a substrate compared to the wild type enzyme | Methylosinus trichosporium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylosinus trichosporium | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
biphenyl + NAD(P)H + H+ + O2 | - |
Methylosinus trichosporium | 2-hydroxybiphenyl + 4-hydroxybiphenyl + NAD(P)+ + H2O | - |
? | |
ethylbenzene + NAD(P)H + H+ + O2 | - |
Methylosinus trichosporium | 1-phenylethanol + 3-ethylphenol + 4-ethylphenol + NAD(P)+ + H2O | - |
? | |
methane + NAD(P)H + H+ + O2 | - |
Methylosinus trichosporium | methanol + NAD(P)+ + H2O | - |
? | |
additional information | inactive toward anthracene and phenanthrene | Methylosinus trichosporium | ? | - |
? | |
naphthalene + NAD(P)H + H+ + O2 | - |
Methylosinus trichosporium | alpha-naphthol + beta-naphthol + NAD(P)+ + H2O | - |
? | |
toluene + NAD(P)H + H+ + O2 | - |
Methylosinus trichosporium | benzyl alcohol + cresol + NAD(P)+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
sMMO | - |
Methylosinus trichosporium |
soluble methane monooxygenase | - |
Methylosinus trichosporium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Methylosinus trichosporium | |
NADPH | - |
Methylosinus trichosporium |