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Literature summary for 1.14.13.25 extracted from

  • Shaofeng, H.; Shuben, L.; Jiayin, X.; Jianzhong, N.; Chungu, X.; Haidong, T.; Wei, T.
    Purification and biochemical characterization of soluble methane monooxygenase hydroxylase from Methylosinus trichosporium IMV 3011 (2007), Biosci. Biotechnol. Biochem., 71, 122-129.
    View publication on PubMed
Search for more literature for 1.14.13.25

Cloned(Commentary)

Cloned (Comment) Organism
phylogenetic analysis Methylosinus trichosporium

General Stability

General Stability Organism
instability of the enzyme during purification is probably due to loss of iron Methylosinus trichosporium

Inhibitors

Inhibitors Comment Organism Structure
Dithionite 34% inhibition at 5 mM Methylosinus trichosporium
DTT 24% inhibition at 20 mM Methylosinus trichosporium
glycerol 40% inhibition at 15% Methylosinus trichosporium
N2 N2 atmosphere causes 28% inhibition Methylosinus trichosporium

Metals/Ions

Metals/Ions Comment Organism Structure
Iron non-heme iron, 3.02 mol iron per mol of enzyme, addition of exogenous FeCl2 or FeCl3 does not affect the enzyme activity Methylosinus trichosporium
additional information the enzyme contains very low or no amounts of copper and zinc Methylosinus trichosporium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
 2 * 58000, alpha-subunit, + 2 * 36000, beta-subunit, + 2 * 23000, gamma-subunit, (alphabetagamma)2, SDS-PAGE Methylosinus trichosporium
36000
-
 2 * 58000, alpha-subunit, + 2 * 36000, beta-subunit, + 2 * 23000, gamma-subunit, (alphabetagamma)2, SDS-PAGE Methylosinus trichosporium
58000
-
 2 * 58000, alpha-subunit, + 2 * 36000, beta-subunit, + 2 * 23000, gamma-subunit, (alphabetagamma)2, SDS-PAGE Methylosinus trichosporium
201300
-
 gel filtration Methylosinus trichosporium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methane + NAD(P)H + O2 Methylosinus trichosporium
-
 methanol + NAD(P)+ + H2O
-
 ?
methane + NAD(P)H + O2 Methylosinus trichosporium IMV 3011
-
 methanol + NAD(P)+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Methylosinus trichosporium
-
-
-
Methylosinus trichosporium IMV 3011
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 8.08fold by anion exchange chromatography, ultrafiltration, gel filtration, and again anion exchange chromatography, to 95% purity Methylosinus trichosporium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.604
-
 purified enzyme, substrate propylene Methylosinus trichosporium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methane + NAD(P)H + O2
-
 Methylosinus trichosporium methanol + NAD(P)+ + H2O
-
 ?
methane + NAD(P)H + O2
-
 Methylosinus trichosporium IMV 3011 methanol + NAD(P)+ + H2O
-
 ?
propylene + NADH + O2
-
 Methylosinus trichosporium propylene epoxide + NAD+ + H2O
-
 ?
propylene + NADH + O2
-
 Methylosinus trichosporium IMV 3011 propylene epoxide + NAD+ + H2O
-
 ?

Subunits

Subunits Comment Organism
hexamer 2 * 58000, alpha-subunit, + 2 * 36000, beta-subunit, + 2 * 23000, gamma-subunit, (alphabetagamma)2, SDS-PAGE Methylosinus trichosporium
More the enzyme mainly exists of alpha-helical regions, circular dichroism measurement Methylosinus trichosporium

Synonyms

Synonyms Comment Organism
methane monooxygenase hydroxylase
-
 Methylosinus trichosporium
sMMO
-
 Methylosinus trichosporium
soluble methane monooxygenase hydroxylase
-
 Methylosinus trichosporium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Methylosinus trichosporium

Cofactor

Cofactor Comment Organism Structure
NAD(P)H
-
 Methylosinus trichosporium

pI Value

Organism Comment pI Value Maximum pI Value
Methylosinus trichosporium isoelectric focusing
-
 5.2