BRENDA - Enzyme Database
show all sequences of 1.14.13.247

Quaternary ammonium oxidative demethylation X-ray crystallographic, resonance Raman, and UV-visible spectroscopic analysis of a Rieske-type demethylase

Daughtry, K.; Xiao, Y.; Stoner-Ma, D.; Cho, E.; Orville, A.; Liu, P.; Allen, K.; J. Am. Chem. Soc. 134, 2823-2834 (2012)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21(DE3) cells
Sinorhizobium meliloti
Crystallization (Commentary)
Crystallization (Commentary)
Organism
in complex with stachydrine, hanging drop vapor diffusion method, using 15% (w/v) poly(ethylene glycol) 3350, 20% (v/v) glycerol, 100 mM succinic acid buffer, pH 7.0, and 10 mM hexamine cobalt at 22C
Sinorhizobium meliloti
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
stachydrine + NADH + H+ + O2
Sinorhizobium meliloti
-
N-methyl-L-proline + formaldehyde + NAD+ + H2O
-
-
?
stachydrine + NADPH + H+ + O2
Sinorhizobium meliloti
-
N-methyl-L-proline + formaldehyde + NADP+ + H2O
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Sinorhizobium meliloti
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Strep-Tactin resin column chromatography
Sinorhizobium meliloti
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
no activity with N,N-dimethyl-glycine, N-methyl-glycine, and N-methyl-L-proline
757058
Sinorhizobium meliloti
?
-
-
-
-
stachydrine + NADH + H+ + O2
-
757058
Sinorhizobium meliloti
N-methyl-L-proline + formaldehyde + NAD+ + H2O
-
-
-
?
stachydrine + NADPH + H+ + O2
-
757058
Sinorhizobium meliloti
N-methyl-L-proline + formaldehyde + NADP+ + H2O
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
stachydrine demethylase
-
Sinorhizobium meliloti
stc2
-
Sinorhizobium meliloti
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
non-heme enzyme
Sinorhizobium meliloti
NADH
-
Sinorhizobium meliloti
NADPH
-
Sinorhizobium meliloti
[2Fe-2S]-center
-
Sinorhizobium meliloti
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Sinorhizobium meliloti
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
non-heme enzyme
Sinorhizobium meliloti
NADH
-
Sinorhizobium meliloti
NADPH
-
Sinorhizobium meliloti
[2Fe-2S]-center
-
Sinorhizobium meliloti
Crystallization (Commentary) (protein specific)
Crystallization
Organism
in complex with stachydrine, hanging drop vapor diffusion method, using 15% (w/v) poly(ethylene glycol) 3350, 20% (v/v) glycerol, 100 mM succinic acid buffer, pH 7.0, and 10 mM hexamine cobalt at 22C
Sinorhizobium meliloti
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
stachydrine + NADH + H+ + O2
Sinorhizobium meliloti
-
N-methyl-L-proline + formaldehyde + NAD+ + H2O
-
-
?
stachydrine + NADPH + H+ + O2
Sinorhizobium meliloti
-
N-methyl-L-proline + formaldehyde + NADP+ + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Strep-Tactin resin column chromatography
Sinorhizobium meliloti
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
no activity with N,N-dimethyl-glycine, N-methyl-glycine, and N-methyl-L-proline
757058
Sinorhizobium meliloti
?
-
-
-
-
stachydrine + NADH + H+ + O2
-
757058
Sinorhizobium meliloti
N-methyl-L-proline + formaldehyde + NAD+ + H2O
-
-
-
?
stachydrine + NADPH + H+ + O2
-
757058
Sinorhizobium meliloti
N-methyl-L-proline + formaldehyde + NADP+ + H2O
-
-
-
?
Other publictions for EC 1.14.13.247
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
757552
Kumar
Prediction and biochemical de ...
Paracoccus denitrificans, Luteovulum sphaeroides, Luteovulum sphaeroides 2.4.1, Paracoccus denitrificans Pd1222
mBio
5
e00933-13
2014
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757058
Daughtry
Quaternary ammonium oxidative ...
Sinorhizobium meliloti
J. Am. Chem. Soc.
134
2823-2834
2012
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