Literature summary for 1.14.13.244 extracted from

Cafaro, V.; Izzo, V.; Scognamiglio, R.; Notomista, E.; Capasso, P.; Casbarra, A.; Pucci, P.; Di Donato, A.
Phenol hydroxylase and toluene/o-xylene monooxygenase from Pseudomonas stutzeri OX1 interplay between two enzymes (2004), Appl. Environ. Microbiol., 70, 2211-2219 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pseudomonas stutzeri

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00061	-	phenol	25°C, pH not specified in the publication	Pseudomonas stutzeri

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas stutzeri	Q84AQ4 and Q84AQ3 and Q84AQ2 and Q84AQ1 and Q84AQ0	Q84AQ4 i.e. subunit PhL, Q84AQ3 i.e. subunit PhM, Q84AQ2 i.e. subunit PhN, Q84AQ1 i.e. subunit PhO, Q84AQ0 i.e. subunit PhP	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
benzene + NADH + H+ + O2	-	Pseudomonas stutzeri	phenol + NAD+ + H2O	-	?
phenol + NADH + H+ + O2	-	Pseudomonas stutzeri	catechol + NAD+ + H2O	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.286	-	phenol	25°C, pH not specified in the publication	Pseudomonas stutzeri

General Information

General Information	Comment	Organism
physiological function	the PhP component transfers electrons from NADH to a subcomplex endowed with hydroxylase activity. Subunit PhM displays a regulatory function. Cupling between phenol hydroxylase and toluene/o-xylene monooxygenase optimizes the use of nonhydroxylated aromatic molecules	Pseudomonas stutzeri

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
469	-	phenol	25°C, pH not specified in the publication	Pseudomonas stutzeri

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Release 2023.1 (January 2023)